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J Korean Cancer Assoc > Volume 20(2); 1988 > Article
Journal of the Korean Cancer Association 1988;20(2): 105-117.
무혈청 규정 배지를 이용한 SNUH 세포주 수립 및 혈청 첨가 배지를 이용한 SNU 세포주 수립에 관한 연구
박재갑, 권남숙, 김진복, 오승근, 이건욱, 최국진, 김수태, 방영주, 김로경, 강순범, 신면우, 이상훈, 김주현, 이종욱
Establishment of SNUH Cell Lines in Serum - free Defined Medium and SNU Cell Lines in Serum Supplemented Medium
Jae Gahb Park, Nam Sook Kwon, Jin Pok Kim, Seung Keun Oh, Kuhn Uk Lee, Kuk Jin Choe, Soo Tae Kim, Yung Jue Bang, Noe Kyeong Kim, Soon Beom Kang, Myon Woo Shin, Sang Hoon Lee, Joo Hyun Kim, Chong Wook
ABSTRACT
Serum-free, defined medium ACL-4 has proven to be useful for the establishment and maintenance of human colorectal carcinoma and lung adenocarcinoma cell lines. To determine the general usefulness of ACL-4 as a medium for establishing cell lines, we compared the growth of cells from many different tumor specimens in R10, ACL-4 and AR5 (ACL-4 plus 5% FBS). From June 1987 to July 1988, 69 specimens from a wide variety of human tumors were cultured in R10 and ACL-4 or AR5. Eleven cancer cell lines were established ...... seven cell lines (4 stomach carcinoma, 1 rectal carcinoma, 1 ovarian carcinoma, 1 osteosarcoma) in R10; two cell lines (1 uterine cervical cancer, 1 laryngeal carcinoma) in ACL-4; and two cell lines in AR5 (1 colon carcinoma, 1 rectal carcinoma) (Table I & 2). Of interest, two B lymphoblastoid (non-neoplastic) cell iines were established from a stomach cancer and an acute myeloblastic leukemia specimen grown in ACL-4. We have found that sodium pyruvate, transferrin, and insulin were very important for the growth of uterine cervical cancer cells. Early in this study, we also found that fibroblast growth was effectively suppressed by culturing tumor cells in the serum free medium ACL-4. Therefore, in later attempts, when possible loss of cultures by fibroblast overgrowth was observed, serum containing media were replaced by ACL-4 to suppress and eventually eliniinate the fibroblast contamination. Although we were able tu establish only 11 new cancer cell lines from 69 specimens, our study has extended the use of ACL-4 medium with or without serum for the growth of uterine cervical and laryngeal cancer cells.
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