Cancer Research and Treatment

Original Article

Clinico-pathologic Parameters for Prediction of Microsatellite Instability in Colorectal Cancer: Sang-Bong Jung, Han-IL Lee, Hoon-Kyu Oh, Im-Hee Shin, Chang-Ho Jeon; Cancer Res Treat. 2012;44(3):179-186. Published online September 30, 2012; DOI: https://doi.org/10.4143/crt.2012.44.3.179

PURPOSE
Although the incidence of microsatellite instability (MSI) accounts for 10-15% of cases of colorectal cancer, its clinical application for all colorectal cancers has widened. We attempted to identify clinical and pathological parameters that may be helpful in selection of patients with MSI-high (MSI-H).
MATERIALS AND METHODS
A total of 120 resected colorectal cancers were enrolled retrospectively for this MSI study. Polymerase chain reaction (PCR) and denaturing high performance liquid chromatography and/or real time PCR methods with five markers and immunohistochemistry (IHC) for MLH1 and MSH2 were performed for analysis of cancer and blood specimens. Clinico-pathologic parameters, including IHC, were investigated in order to determine their usefulness as predictive factors of MSI.
RESULTS
Among 120 cases of colorectal cancer, MSI was observed in 15 cases (12.5%), including 11 cases of MSI-H and four cases of MSI-low. Patients with MSI were younger, less than 50 years old, had a family history of cancer, Rt. sided colon cancer and/or synchronous multiple colorectal cancer, mucinous histologic type, and serum carcinoembryonic antigen group in the normal range. Results of multivariate analysis showed Bethesda guidelines, Rt. sided and/or synchronous multiple colorectal cancer, and negative expression of IHC for MLH1, which was consistently associated with MSI-H. MSI-H colorectal tumors have met at least one of these three parameters and their sensitivity and specificity were 100% and 72.5%, respectively.
CONCLUSION
Bethesda guidelines, tumor location, and negative expression of MLH1 protein are important parameters for selection of patients with colorectal cancers for MSI testing. MSI testing is recommended for patients showing any of these three parameters.

Citations

Citations to this article as recorded by

Predicting mechanism of immune response in microsatellite instability colorectal cancer
Peng Sun, Yusong Luan, Xuhao Cai, Qi Liu, Peide Ren, Pengpan Xin, Yonggang Yu, Bolun Song, Yangyang Wang, Huijing Chang, Haoyue Ma, Yinggang Chen
Heliyon.2024; 10(6): e28120. CrossRef
Comparison of histopathologic and clinical characteristics of microsatellite instability in colorectal adenocarcinomas and its impact on survival
Bilal Tunçtürk, Ulaş Alabalik, Ayşe Nur Keleş
Biotechnology & Biotechnological Equipment.2024;[Epub] CrossRef
Interventional gastroenterology in oncology
Vaibhav Wadhwa, Nicole Patel, Dheera Grover, Faisal S. Ali, Nirav Thosani
CA: A Cancer Journal for Clinicians.2023; 73(3): 286. CrossRef
Molecular profiling of the colon cancer in South-Eastern Romania
Razvan Catalin Popescu, Cristina Tocia, Costel Brînzan, Georgeta Camelia Cozaru, Mariana Deacu, Andrei Dumitru, Nicoleta Leopa, Anca Florentina Mitroi, Anca Nicolau, Eugen Dumitru
Medicine.2021; 100(1): e24062. CrossRef
Potential Mechanism of Immune Evasion Associated with the Master Regulator ASCL2 in Microsatellite Stability in Colorectal Cancer
Qian Yang, Guowei Huang, Liyan Li, Enmin Li, Liyan Xu, Carlo Cavaliere
Journal of Immunology Research.2021; 2021: 1. CrossRef
Genetic and epigenetic analysis of the beta-2-microglobulin gene in microsatellite instable colorectal cancer
Zuzana Snahnicanova, Ivana Kasubova, Michal Kalman, Marian Grendar, Peter Mikolajcik, Eva Gabonova, Ludovit Laca, Martin Caprnda, Luis Rodrigo, Rachele Ciccocioppo, Peter Kruzliak, Lukas Plank, Zora Lasabova
Clinical and Experimental Medicine.2020; 20(1): 87. CrossRef
Expression and significance of miR-654-5p and miR-376b-3p in patients with colon cancer
Ping Li, Jia-Xun Cai, Fei Han, Jie Wang, Jia-Jie Zhou, Kai-Wen Shen, Liu-Hua Wang
World Journal of Gastrointestinal Oncology.2020; 12(4): 492. CrossRef
Concomitant occurrence of primary renal non-Hodgkin lymphoma and a colon cancer
Ji Li, Yabin Zou, Bin Wang, Xiangwei Meng, Xun Sun
Medicine.2019; 98(10): e14802. CrossRef
Meta-analysis of the molecular associations of mucinous colorectal cancer
I S Reynolds, S J Furney, E W Kay, D A McNamara, J H M Prehn, J P Burke
British Journal of Surgery.2019; 106(6): 682. CrossRef
Association between clinicopathological characteristics and RAS mutation in colorectal cancer
Johan Rimbert, Gaëlle Tachon, Audelaure Junca, Claire Villalva, Lucie Karayan-Tapon, David Tougeron
Modern Pathology.2018; 31(3): 517. CrossRef
Deficient mismatch repair and RAS mutation in colorectal carcinoma patients: a retrospective study in Eastern China
Xiangyan Zhang, Wenwen Ran, Jie Wu, Hong Li, Huamin Liu, Lili Wang, Yujing Xiao, Xiaonan Wang, Yujun Li, Xiaoming Xing
PeerJ.2018; 6: e4341. CrossRef
Comparison of the eighth version of the American Joint Committee on Cancer manual to the seventh version for colorectal cancer: A retrospective review of our data
Guo-Jun Tong, Gui-Yang Zhang, Jian Liu, Zhao-Zheng Zheng, Yan Chen, Ping-Ping Niu, Xu-Ting Xu
World Journal of Clinical Oncology.2018; 9(7): 148. CrossRef
Analysis of factors influencing molecular testing at diagnostic of colorectal cancer
Quentin Thiebault, Gautier Defossez, Lucie Karayan-Tapon, Pierre Ingrand, Christine Silvain, David Tougeron
BMC Cancer.2017;[Epub] CrossRef
Genetic heterogeneity in synchronous colorectal cancers impacts genotyping approaches and therapeutic strategies
Moritz Jesinghaus, Nicole Pfarr, Matthias Kloor, Volker Endris, Luca Tavernar, Alexander Muckenhuber, Magnus von Knebel Doeberitz, Roland Penzel, Wilko Weichert, Albrecht Stenzinger
Genes, Chromosomes and Cancer.2016; 55(3): 268. CrossRef
The Immune Biology of Microsatellite-Unstable Cancer
Matthias Kloor, Magnus von Knebel Doeberitz
Trends in Cancer.2016; 2(3): 121. CrossRef
Stromal expression of miR-21 in T3-4a colorectal cancer is an independent predictor of early tumor relapse
Won Kyung Kang, Jin Kwon Lee, Seong Taek Oh, Sung Hak Lee, Chan Kwon Jung
BMC Gastroenterology.2015;[Epub] CrossRef
KRASandBRAFgene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients
Ju-Xiang Ye
World Journal of Gastroenterology.2015; 21(5): 1595. CrossRef
Colorectal cancer
Hermann Brenner, Matthias Kloor, Christian Peter Pox
The Lancet.2014; 383(9927): 1490. CrossRef
AJCC Cancer Staging Manual 7th Edition Criteria for Colon Cancer: Do the Complex Modifications Improve Prognostic Assessment?
Danielle M. Hari, Anna M. Leung, Ji-Hey Lee, Myung-Shin Sim, Brooke Vuong, Connie G. Chiu, Anton J. Bilchik
Journal of the American College of Surgeons.2013; 217(2): 181. CrossRef

11,610 View
94 Download
19 Crossref

First
Prev
Page of 1
Next
Last

Search