Cancer Research and Treatment

Original Articles

Clinico-pathologic Parameters for Prediction of Microsatellite Instability in Colorectal Cancer: Sang-Bong Jung, Han-IL Lee, Hoon-Kyu Oh, Im-Hee Shin, Chang-Ho Jeon; Cancer Res Treat. 2012;44(3):179-186. Published online September 30, 2012; DOI: https://doi.org/10.4143/crt.2012.44.3.179

PURPOSE
Although the incidence of microsatellite instability (MSI) accounts for 10-15% of cases of colorectal cancer, its clinical application for all colorectal cancers has widened. We attempted to identify clinical and pathological parameters that may be helpful in selection of patients with MSI-high (MSI-H).
MATERIALS AND METHODS
A total of 120 resected colorectal cancers were enrolled retrospectively for this MSI study. Polymerase chain reaction (PCR) and denaturing high performance liquid chromatography and/or real time PCR methods with five markers and immunohistochemistry (IHC) for MLH1 and MSH2 were performed for analysis of cancer and blood specimens. Clinico-pathologic parameters, including IHC, were investigated in order to determine their usefulness as predictive factors of MSI.
RESULTS
Among 120 cases of colorectal cancer, MSI was observed in 15 cases (12.5%), including 11 cases of MSI-H and four cases of MSI-low. Patients with MSI were younger, less than 50 years old, had a family history of cancer, Rt. sided colon cancer and/or synchronous multiple colorectal cancer, mucinous histologic type, and serum carcinoembryonic antigen group in the normal range. Results of multivariate analysis showed Bethesda guidelines, Rt. sided and/or synchronous multiple colorectal cancer, and negative expression of IHC for MLH1, which was consistently associated with MSI-H. MSI-H colorectal tumors have met at least one of these three parameters and their sensitivity and specificity were 100% and 72.5%, respectively.
CONCLUSION
Bethesda guidelines, tumor location, and negative expression of MLH1 protein are important parameters for selection of patients with colorectal cancers for MSI testing. MSI testing is recommended for patients showing any of these three parameters.

Citations

Citations to this article as recorded by

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Comparison of histopathologic and clinical characteristics of microsatellite instability in colorectal adenocarcinomas and its impact on survival
Bilal Tunçtürk, Ulaş Alabalik, Ayşe Nur Keleş
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Vaibhav Wadhwa, Nicole Patel, Dheera Grover, Faisal S. Ali, Nirav Thosani
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Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid: Kwan-Ho Lee, Kyung-Chul Shin, Chae-Hun Lee, Sang-Hoon Jheon, Chang-Ho Jeon; Cancer Res Treat. 2007;39(2):69-73. Published online June 30, 2007; DOI: https://doi.org/10.4143/crt.2007.39.2.69

Abstract PDF PubReader ePub

Purpose

Bronchial wash fluid may be a useful for detecting lung cancer. To increase the detection rates, we performed molecular analysis with using MAGE A1-6 and SSX4 RT-PCR on bronchial wash fluid specimens.

Materials and Methods

We obtained 57 lung cancer tissue specimens by bronchoscopic biopsy and 131 bronchial washes from 96 patients with lung cancer and 35 patients with benign lung diseases. The MAGE A1-6 and SSX4 gene expressions were investigated in the cancer tissue specimens and bronchial wash fluids. We evaluated the positive detection rates of these methods according to the cytology results and the clinical findings.

Results

For the cancer tissue specimens and the bronchial wash fluid, the positive detection rate of MAGE or SSX4 was 91.2% and 75.0%, respectively. Combined MAGE and SSX4 PCR and cytology tests showed an 83.3% detection rate for the bronchial wash fluid. From bronchial washes of patients with benign lung diseases, the positive rates of using MAGE or SSX4 was 11.4%. In the bronchial wash fluid of lung cancer patients, 66.7% of the peripheral cancers were detected by MAGE or SSX4, while examination with cytology did not detect any peripheral lung cancer.

Conclusion

The application of both MAGE and SSX4 showed high sensitivity and specificity for the detection of lung cancer. Thus, MAGE and SSX4 RT-PCR may be effectively utilized as additional methods to improve detection of lung cancer with using bronchial wash fluids.

Citations

Citations to this article as recorded by

Characterization of Melanoma-Associated Antigen-A Genes Family Differential Expression in Non-Small-Cell Lung Cancers
Shirin Karimi, Foroozan Mohammadi, Mihan Porabdollah, Seyed Amir Mohajerani, Kian Khodadad, Seyed Alireza Nadji
Clinical Lung Cancer.2012; 13(3): 214. CrossRef
Expression of MAGE A 1-6 and SSX 1-9 Genes in the Sputum and Cancer Tissue of the Lung Cancer Patients
Yeun Jae Lee, Jang Hoon Lee, Jung Cheul Lee, Kwan Ho Lee
Tuberculosis and Respiratory Diseases.2011; 70(4): 315. CrossRef

9,576 View
44 Download
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