| Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines |
| Sang Uk Han, Won Hung Lee, Wook Hwan Kim, Myung Wook Kim, Jae Ho Lee, Sang Yong Song, Kuhn Uk Lee |
1Departments of Surgery, School of Medicine, Ajou University, Suwon, Korea. hansu@madang.ajou.ac.kr
2Departments of Biochemistry, School of Medicine, Ajou University, Suwon, Korea.
3Department of Diagnostic Pathology, School of Medicine, Sung Kyun Kwan University,Samsung Medical Center, Seoul, Korea.
4Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.
|
|
|
|
|
|
| ABSTRACT |
PURPOSE:
Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied.
MATERIALS AND METHODS:
Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells.
Tyrosine phosphorylation of c-Met, E-cadherin, alpha-, beta-, gamma-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.
RESULTS:
Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.
CONCLUSION:
These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin. |
| Key words:
Stomach neoplasm;Hepatocyte growth factor;c-Met gene;E-cadherin;Invasion |
|
PDF Links
Full text via DOI 
Download Citation 
