| Home | E-Submission | Sitemap | Contact Us |  
J Korean Cancer Assoc > Volume 32(5); 2000 > Article
Journal of the Korean Cancer Association 2000;32(5): 981-988.
K-ras Mutation in Ampulla of Vater Cancer
Sang Jae Park, Sun Whe Kim, Woo Ho Kim, Jin Young Jang, Yong Hyun Park
1Departments of Surgery, Seoul National University College of Medicine, Seoul, Korea.
2Departments of Pathology, Seoul National University College of Medicine, Seoul, Korea.
The aims of this study was to elucidate the role of the K-ras mutation (codon 12) in the carcinogenesis of ampulla of Vater cancer.
31 cases of radically resected ampullary cancer cases were enrolled. 18 cases harbored the adenomatous portions adjacent to carcinomatous portions. Utilizing a two-step nested PCR with RFLP method, we examined the K-ras mutation in a total of 90 samples (normal mucosas (N=31), carcinomatous portions (N=31), adenomatous portions (N=18), and metastatic lymph nodes (N=10)).
In the carcinomatous portion, K-ras mutations in codon 12 were detected in 48.4%. In the adenomatous portions, 10 cases (55.6%) out of 18 displayed the K-ras mutation. In the metastatic lymph nodes, K-ras mutation was found in 4 cases out of 10. After sequencing, 4 mutant types (GTT, GAT, GCT and TGT) of codon 12 (normally GGT) in the ampulla of Vater cancer were detected and the mutated types in the adenomatous portions, carcinomatous portions and metastatic lymph nodes in the same cases were identical.
The presence of concomitant K-ras mutation in both the adenomatous and carcinomatous portions in some cases may suggest the role of K-ras mutation in the early carcinogenesis of ampulla of Vater cancer.
Key words: Ampulla of Vater neoplasm;K-ras gene;Mutation;Carcinogenesis
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Related articles
Radiation Therapy of Laryngeal Cancer  1982 ;14(1)
Regulation and Role of EZH2 in Cancer  2014 July;46(3)
Combined Resection in Advanced Gastric Cancer  1999 June;31(3)
RNA Regulation in Neurologic Disease and Cancer  2010 September;42(3)
Editorial Office
Korean Cancer Association
Room 1824, Gwanghwamun Officia
92 Saemunan-ro, Jongno-gu, Seoul 03186, Korea
TEL: +82-2-3276-2410   FAX: +82-2-792-1410   E-mail: journal@cancer.or.kr
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © Korean Cancer Association.                 Developed in M2PI