| Home | E-Submission | Sitemap | Contact Us |  
top_img
Cancer Research and Treatment > Volume 34(6); 2002 > Article
Cancer Research and Treatment 2002;34(6): 444-449. doi: https://doi.org/10.4143/crt.2002.34.6.444
Antisense Deoxyoligonucleotides Inhibit Activities of Matrix Metalloproteinase-2 in Human Fibrosarcoma HT1080 Cells
Jung Sun Park, Dong On Yang, Seon Hee Lim, Hyeon Gyeong Yoo, Heyon Na Cho, Young Do Jung, Sae Jong Kim, Sun Sik Chung, Boo Ahn Shin
Research Institute of Medical Sciences, Chonnam NationalUniversity, Gwangju, Korea. bashin@chonnam.ac.kr
  Published online: December 31, 2002.
ABSTRACT
PURPOSE:
MMP-2, 72 kDa-type IV collagenase, plays a major role in the migration and growth of tumor cells, a process that requires the disintegration of basement membrane. Activation of MMP-2 is correlated with the invasiveness of various tumors. The aim of this study was to determine the sequence-specific phosphorothioated oligodeoxynucleotides (ODNs) inhibiting the translation of MMP-2 mRNA and the subsequent invasiveness of tumor cells.
MATERIALS AND METHODS:
Eight types of antisense ODNs were designed and each (8micro gram/ml) were transfected into HT1080 cells. The effects of these antisense ODNs on MMP expression were examined by gelatin zymography, Western blot, Northern blot and matrigel assay.
RESULTS:
Antisense-5 (+904~923), antisense-6 (+1274~+1293) and antisense-7 (+1646~+1665) reduced the MMP-2 activity of the culture supernatant in HT1080 fibrosarcoma cells. Treatment with antisense-6 showed inhibition of MMP-2 mRNA and protein, and in vitro invasion in a dose-dependent manner.
CONCLUSION:
Antisense-6 might be one of the therapeutic candidates for tumor invasion and metastasis.
Key words: Matrix metalloproteinases-2;Antisense oligodeoxynucleotides;Tumor invasion;Metastasis
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Share:      
METRICS
0
Crossref
0
Scopus
3,365
View
18
Download
Related article
Editorial Office
Korean Cancer Association
Room 1824, Gwanghwamun Officia
92 Saemunan-ro, Jongno-gu, Seoul 03186, Korea
TEL: +82-2-3276-2410   FAX: +82-2-792-1410   E-mail: journal@cancer.or.kr
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © Korean Cancer Association.                 Developed in M2PI