| Gene Expression Profiling of Non-Small Cell Lung Cancer |
| Mee Sook Roh, Hyuk Chan Kwon, Jin Sook Jeong, Dae Cheol Kim, Choon Hee Son, Soo Keol Lee, Phil Jo Choi, Jae Ik Lee, Ki Nam Lee, Hyo Jin Kim, Jin Han Yoon, Tae Ho Hwang |
1Department of Pathology, Dong-A University College ofMedicine, Busan, Korea.
2Department of Internal Medicine, Dong-A University Collegeof Medicine, Busan, Korea.
3Department of Thoracic and Cardiovascular Surgery, Dong-AUniversity College of Medicine, Busan, Korea.
4Department of Diagnostic Radiology, Dong-A UniversityCollege of Medicine, Busan, Korea.
5Department of Urology, Dong-A University College ofMedicine, Busan, Korea.
6Department of Pharmacology, Dong-A University College ofMedicine, Busan, Korea. thhwang@mail.donga.ac.kr
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Published online: April 30, 2003. |
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| ABSTRACT |
PURPOSE:
cDNA microarray provided a powerful alternative, with an unprecedented view scope, in monitoring gene expression levels, and led to the discovery of regulatory pathways involved in complicated biological processes. This study was performed to gain better understanding of the molecular mechanisms underlying the carcinogenesis and progression of lung cancer.
MATERIALS AND METHODS:
Using a cDNA microarray, representing 4, 600 cDNA clusters, we studied the expression profiles in 10 non-small cell lung cancer (NSCLC) samples and the adjacent noncancerous lung tissues form the same patients. The alterations in the levels of gene expression were confirmed by reverse-transcription PCR in 10 randomly selected genes.
RESULTS:
Genes that were differently expressed in the cancerous and noncancerous tissues were identified. One hundred and nine genes (of which 68 were known) and 69 cDNAs (of which 32 were known) were up- and down-regulated in>70% of the NSCLC samples, respectively. In the cancerous tissues, the genes related to the cell cycle, metabolism, cell structure and signal transduction, were mostly up-regulated. Furthermore, we identified a few putative tumor suppressor genes that had previously been proposed by other workers.
CONCLUSIONS:
These results provide, not only a new molecular basis for understanding the biological properties of NSCLC, but also useful resources for the future development of diagnostic markers and therapeutic targets for NSCLC. |
| Key words:
cDNA microarray;Gene expression;Non- small cell lung cancer |
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