Assessing the Efficacy of Bortezomib and Dexamethasone for Induction and Maintenance Therapy in Relapsed/Refractory Cutaneous T-Cell Lymphoma: A Phase II CISL1701/BIC Study

Yoon Seok Choi; Joonho Shim; Ka-Won Kang; Sang Eun Yoon; Jun Sik Hong; Sung Nam Lim; Ho-Young Yhim; Jung Hye Kwon; Gyeong-Won Lee; Deok-Hwan Yang; Sung Yong Oh; Ho-Jin Shin; Hyeon-Seok Eom; Dok Hyun Yoon; Hong Ghi Lee; Seong Hyun Jeong; Won Seog Kim; Seok Jin Kim

doi:10.4143/crt.2024.479

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Original Article Hematologic malignancy Assessing the Efficacy of Bortezomib and Dexamethasone for Induction and Maintenance Therapy in Relapsed/Refractory Cutaneous T-Cell Lymphoma: A Phase II CISL1701/BIC Study: Yoon Seok Choi¹, Joonho Shim², Ka-Won Kang¹, Sang Eun Yoon³, Jun Sik Hong⁴, Sung Nam Lim⁵, Ho-Young Yhim⁶, Jung Hye Kwon⁷, Gyeong-Won Lee⁸, Deok-Hwan Yang⁹, Sung Yong Oh¹⁰, Ho-Jin Shin¹¹, Hyeon-Seok Eom¹², Dok Hyun Yoon¹³, Hong Ghi Lee¹⁴, Seong Hyun Jeong¹⁵, Won Seog Kim³, Seok Jin Kim^3,; Cancer Research and Treatment : Official Journal of Korean Cancer Association 2025;57(1):267-279.
DOI: https://doi.org/10.4143/crt.2024.479
Published online: July 16, 2024

¹Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea

²Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

³Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

⁴Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea

⁵Department of Internal Medicine, Inje University Haeundae Paik Hospital, Busan, Korea

⁶Department of Internal Medicine, Jeonbuk National University Medical School, Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Korea

⁷Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Korea

⁸Division of Hematology and Oncology, Department of Internal Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Changwon, Korea

⁹Department of Internal Medicine, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Hwasun, Korea

¹⁰Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea

¹¹Department of Internal Medicine, Pusan National University College of Medicine, Busan, Korea

¹²Department of Hematology-Oncology, Center for Hematologic Malignancy, National Cancer Center, Goyang, Korea

¹³Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

¹⁴Department of Internal Medicine, Konkuk University Hospital, Seoul, Korea

¹⁵Department of Hematology-Oncology, Ajou University School of Medicine, Suwon, Korea

Correspondence: Seok Jin Kim, Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: 82-2-3410-1766 Fax: 82-2-3410-1754 E-mail: kstwoh@skku.edu

*Yoon Seok Choi and Joonho Shim contributed equally to this work.

• Received: May 21, 2024 • Accepted: July 15, 2024

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract

Purpose
This multicenter, open-label, phase II trial evaluated the efficacy and safety of bortezomib combined with dexamethasone for the treatment of relapsed/refractory cutaneous T-cell lymphoma (CTCL) in previously treated patients across 14 institutions in South Korea.
Materials and Methods
Between September 2017 and July 2020, 29 patients with histologically confirmed CTCL received treatment, consisting of eight 4-week cycles of induction therapy followed by maintenance therapy, contingent upon response, for up to one year. The primary endpoint was the proportion of patients achieving an objective global response.
Results
Thirteen of the 29 patients (44.8%) achieved an objective global response, including two complete responses. The median progression-free survival (PFS) was 5.8 months, with responders showing a median PFS of 14.0 months. Treatment-emergent adverse events were generally mild, with a low incidence of peripheral neuropathy and hematologic toxicities. Despite the trend toward shorter PFS in patients with higher mutation burdens, genomic profiling before and after treatment showed no significant emergence of new mutations indicative of disease progression.
Conclusion
This study supports the use of bortezomib and dexamethasone as a viable and safe treatment option for previously treated CTCL, demonstrating substantial efficacy and manageability in adverse effects. Further research with a larger cohort is suggested to validate these findings and explore the prognostic value of mutation profiles.
Key words: Cutaneous T-cell lymphoma, Bortezomib, Dexamethasone, Relapsed/refractory, Treatment efficacy, Safety profile

Introduction

Cutaneous T-cell lymphomas (CTCLs) comprise a heterogeneous group of rare extranodal lymphoproliferative disorders primarily affecting the skin, stemming from tissue-resident T cells [1]. Among the various histologic subtypes of CTCLs, the most prevalent is mycosis fungoides (MF) [2]. Treatment approaches for CTCLs are tailored according to the disease stage [3]. Early-stage CTCLs (patch/plaque stage) typically demonstrate an indolent clinical course with an excellent prognosis and can often be effectively managed using skin-directed therapies over an extended period [4]. The therapeutic goal is to alleviate symptoms and enhance quality of life while minimizing treatment-related adverse effects. Conversely, advanced-stage CTCLs tend to follow a progressive clinical course with a poorer prognosis, often necessitating systemic treatment [5]. Advanced diseases commonly exhibit rapid relapse and progression, even following an initial response to systemic therapies, thereby limiting the utility of combination chemotherapy with cytotoxic agents. Consequently, frontline systemic therapy for CTCLs often favors less toxic biologics such as retinoid derivatives and IFN-α over cytotoxic agents [6].

Over the past two decades, a range of systemic agents, including less myelosuppressive cytotoxic drugs, histone deacetylase inhibitors, and mammalian target of rapamycin inhibitors, have been evaluated in phase II trials for their effectiveness against CTCLs [7-10]. However, these agents typically demonstrated only modest efficacy, with response rates below 40% and brief durations of response. Recently, following positive outcomes from two phase III trials, brentuximab vedotin and mogamulizumab received approval and have been incorporated into standard practice for treating relapsed/refractory CTCLs [11-13]. Additionally, emerging immunotherapeutic strategies, such as antagonistic antibodies targeting immune checkpoints and chimeric antigen receptor (CAR) T-cell therapies, have shown early signs of effectiveness against CTCLs [14,15]. Despite these advances, nearly all patients ultimately experience disease relapse and progression. Thus, CTCLs are currently regarded as disorders that are difficult or even impossible to cure. Given this clinical reality, expanding the range of available treatment options would be highly beneficial for patients.

Prior preclinical research has underscored the vital role of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in both the proliferation and survival of lymphoma cells [16]. Specifically, activating mutations in TNFR2 (tumor necrosis factor receptor 2) have been found to lead to persistent NF-κB activation in primary tumor cells from patients with CTCL, closely linking to resistance against apoptosis [17]. Consequently, NF-κB emerges as a compelling therapeutic target. Indeed, a recent phase II study demonstrated significant efficacy of dimethyl fumarate, an NF-κB inhibitor, in treating MF and Sézary syndrome (SS) [18]. Furthermore, bortezomib, a proteasome inhibitor used in various lymphoid malignancies, prevents IκB degradation, thereby inhibiting NF-κB activity and exhibiting antitumor effects in CTCL [19].

Therefore, we conducted a phase II study to evaluate the efficacy and safety of bortezomib in combination with high-dose dexamethasone in patients with relapsed or refractory CTCL. This paper presents the results of our study, which includes both pre- and post-treatment targeted sequencing of tumor tissue.

Materials and Methods

1. Participants and eligibility criteria

The research was carried out from 2017 to 2020 across 14 academic institutions throughout South Korea. The study included patients 19 years of age or older who were histologically diagnosed with CTCL. This encompassed several subtypes such as MF, SS, primary cutaneous anaplastic large cell lymphoma, lymphomatoid papulosis, and primary cutaneous peripheral T-cell lymphoma unspecified. Eligibility was limited to those who had not responded to at least one prior skin-directed or systemic therapy. Participants were required to have completed any form of anti-lymphoma treatment at least four weeks prior to enrollment and must have been free from any significant treatment-related toxic effects at the time of entry. Additional inclusion criteria were an Eastern Cooperative Oncology Group (ECOG) performance status ranging from 0 to 2, and sufficient organ and bone marrow functionality, evidenced by a leukocyte count of at least 4,000/μL, an absolute neutrophil count of no less than 1,500/μL, and a platelet count exceeding 100,000/μL. Exclusion criteria included pregnancy or lactation, fertile women not employing effective contraception, and individuals with active infections or a past or concurrent secondary neoplasm.

2. Study design and treatment

This phase II, open-label, single-arm trial evaluates the antitumor efficacy and safety of bortezomib combined with dexamethasone in patients with relapsed or refractory CTCL (ClinicalTrials.gov: NCT03487133). The regimen is depicted in S1 Fig. and comprises two treatment phases: (1) Induction therapy: over eight 4-week cycles, patients receive subcutaneous bortezomib (1.6 mg/m²) and dexamethasone (40 mg) administered intravenously or orally on days 1, 8, and 15 of each cycle. Patients exhibiting disease progression at the end of the fourth or eighth cycle are withdrawn from the trial; (2) Maintenance therapy: following the induction phase, patients who achieve at least stable disease continue into the maintenance phase. This involves receiving the same dosages of bortezomib and dexamethasone on day 1 of each subsequent 4-week cycle, with therapy continuing until disease progression or the emergence of intolerable side effects, up to a maximum duration of 1 year.

3. Efficacy and safety assessments

Efficacy and safety assessments were conducted bi-cyclically throughout the treatment duration. The clinical overall response was quantified using the global composite response score, which integrates responses across four anatomical compartments—skin, lymph nodes, viscera, and blood. This scoring adheres to the consensus guidelines established by the International Society for Cutaneous Lymphomas, the United States Cutaneous Lymphoma Consortium, and the European Organization for Research and Treatment of Cancer (ISCL-USCLC-EORTC) [20,21]. Skin compartment tumor burden was specifically measured utilizing the modified Severity-Weighted Assessment Tool (mSWAT). For patients presenting with lymph node or visceral involvement, whole-body positron emission tomography–computed tomography and/or contrast-enhanced computed tomography scans were performed at screening, after every two treatment cycles (cycles 2, 4, 6, 8, 11, 14, 17, and 21), and at treatment conclusion. Concurrently, patients with hematologic involvement underwent flow cytometry assessments at identical intervals to the skin evaluations. Adverse events were systematically recorded following the National Cancer Institute’s Common Terminology Criteria for Adverse Events (CTCAE), ver. 4.3.

4. Study endpoints

The primary endpoint of the study was to establish the overall response rate (ORR) in accordance with the ISCL-USCLC-EORTC response criteria. Among the secondary endpoints, the proportion of patients achieving an objective global response sustained for at least 4 months (ORR4) was evaluated. Additional secondary endpoints included the disease control rate (DCR), defined as the proportion of patients who achieved stable disease or better; the duration of response (DOR); progression-free survival (PFS); overall survival (OS); and safety metrics.

5. Ethical considerations

The study was conducted in strict compliance with the International Ethical Guidelines for Biomedical Research Involving Human Subjects, Good Clinical Practice (GCP) guidelines, and the Declaration of Helsinki. The study protocol and informed consent documents received approval from the institutional review boards (IRBs) at all participating institutions. Final approval was also obtained from the Korean Ministry of Food and Drug Safety (MFDS). Written informed consent was obtained from all participants prior to their involvement in the study. Additionally, all experimental procedures involving patient-derived specimens and molecular testing were conducted in accordance with the approved IRB protocol.

6. Targeted Sequencing and variant detection

Genomic DNA was isolated from tumor tissues and sheared using a QIAamp DNA Mini kit (Qiagen, Valencia, CA) and Covaris S220 (Covaris, Woburn, MA). The concentration and purity of the DNA were evaluated using a Nanodrop 8000 UV-Vis spectrometer (Thermo Fisher Scientific, Waltham, MA) and a Picogreen fluorescence assay with a Qubit 2.0 Fluorometer (Life Technologies, Foster City, CA). Fragment size distribution was analyzed using a 2200 TapeStation (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instruction (S2 Table). For high-throughput sequencing, HemaSCAN panel, covering the whole exome of 425 genes (S3 Table) linked to hematological malignancies, was employed as previously described [22]. Single-nucleotide variants were called using MuTect version 1.1.4 and Lowfreq ver. 0.6.1 with specific criteria for variant allele frequency set at ≥ 1% or supporting reads greater than four. To enhance sensitivity, the candidate set of variants was formed by combining the variants identified both callers, with the high confidence set from MuTect. Sequencing artifacts were filtered out using a machine learning algorithm analyzing features from the SAM files, as an additional filtering step. Briefly, this process included the application of a logistic regression model trained to discern false-positive calls occurring normal samples, including abnormally aligned, clustered, and strand-biased reads. Additionally, low-confidence variants (supporting reads < 20) were manually inspected using the Integrative Genomics Viewer. Small insertions and deletions were detected using Pindel version 0.2.5a4, with the criterion for variant supporting reads set at > 9. Variants with a minor allele frequency of ≥ 1% in population databases such as the 1000 Genomes Project and the Korean Reference Genome Database were excluded. Copy number variations were assessed, with amplifications defined by a copy number > 5 and deletions by a copy number < 1.2. JuLI was employed to identify deletions greater than 30 bp and structural variants.

7. Statistical methods

The study was designed using Simon’s two-stage phase II optimal design to provide 80% power and maintain a one-sided type I error rate of 0.05. The design aimed to differentiate between a non-effective ORR of 10% and a target ORR of 30%. The initial cohort comprised 10 patients; expansion to 29 patients was contingent upon the occurrence of at least two responders within this group. A positive trial outcome was predetermined if more than 10 out of the 29 patients demonstrated a response. ORR calculations, along with their 95% confidence intervals (CIs), were performed using the Clopper-Pearson exact method. DoR, PFS, and OS were analyzed using the Kaplan-Meier survival estimation technique. Demographic characteristics, other clinical outcomes, and safety data were summarized descriptively. All statistical analyses were conducted using SPSS ver. 25.0 (IBM Corp., Armonk, NY).

Results

1. Demographics and characteristics

Between September 15, 2017, and July 22, 2020, a total of 32 patients were assessed for eligibility. Of these, 29 were enrolled in the phase II study (Fig. 1A). Their demographics and baseline characteristics are detailed in Table 1. The median age of participants was 59 years, with an age range from 26 to 82 years. The cohort included 19 patients diagnosed with MF, six with CD30+ primary cutaneous T-cell lymphoproliferative disorders, and four with unspecified primary cutaneous peripheral T-cell lymphoma. Among the patients with MF, 13 presented with advanced clinical stages (stage IIB or higher) at the start of the study. Patients had a median of two previous lines of systemic therapy before enrollment, with most having been treated with systemic cytotoxic chemotherapy.

2. Treatment duration and efficacy outcomes

As illustrated in Fig. 1A, only three patients (10.3%) completed the entire planned 20-cycle course of treatment. The majority, comprising 26 patients (89.7%), discontinued treatment, resulting in a median treatment duration of five cycles. Among those discontinuing, 22 patients (75.9%) ceased treatment due to disease progression, while three patients (10.3%) withdrew their consent (Fig. 1B). At the data cutoff point (median follow-up of 17.0 months [range, 1.53 to 46.6 months]), the ORR was 44.8% (95% CI, 27.6 to 62.1), with two complete responses noted (Table 2). All responders showed improvement in their skin involvement, with the median score of the best change in mSWAT from baseline being –25%, ranging from 58% to –100% (Fig. 1C). Significant tumor responses were observed particularly in patients with advanced MF, including those with erythrodermic and tumor stage manifestations (Fig. 2A). Proportion of patients with tumor response in extracutaneous compartments is also described in Table 3. The response rate remained consistent across different histologic subtypes. Notably, a sustained response of over 4 months (ORR4) was recorded in 34.5% of cases (95% CI, 17.2 to 51.7). The median DOR among these patients was 8.6 months (Fig. 1B), and the DCR was 69.0% (95% CI, 51.7 to 86.2).

3. Survival analysis

Progression or death occurred in 23 patients (79.3%). The median PFS was calculated at 5.83 months (95% CI, 2.49 to 9.17) (Fig. 2B). Notably, in the 13 patients who responded to treatment, the median PFS extended to 14.0 months (95% CI, 6.85 to 21.15) (Fig. 2C). OS events were recorded in seven patients (24.1%), and the median OS had not been reached at the time of data analysis (Fig. 2D).

4. Safety and tolerability

Throughout the study, no treatment-related fatalities were reported. A significant proportion of the participants, 24 patients (82.8%), experienced at least one treatment-emergent adverse event (TEAE), as detailed in Table 4. The most frequently reported TEAEs included peripheral neuropathy (20.7%), fatigue (17.2%), and skin rash (17.2%). Severe adverse events, categorized as grade 3/4 TEAEs, were relatively uncommon, affecting only four patients (13.8%). This included a singular case (3.4%) of grade 4 thrombocytopenia. Additionally, two patients (6.9%) experienced serious infections; one of these was a case of pneumonia that necessitated hospitalization. Overall, the combination of bortezomib and dexamethasone demonstrated manageable toxicities, with most TEAEs being of grade 1 or 2 severity. According to the study protocol, dose delay and reduction were required in only one case, which was due to grade 4 thrombocytopenia. Importantly, none of the adverse events led to the discontinuation of study treatment.

5. Mutation analysis and genomic landscape comparison

Following enrollment, pre-treatment tissue samples from patients were collected and preserved for targeted sequencing to evaluate the mutation profiles of relapsed or refractory CTCL before starting treatment with bortezomib and dexamethasone (S4 Table). Of the 29 patients enrolled, targeted sequencing was performed on 21 whose tissue samples were available, as shown in Fig. 3A. The most frequent mutations in the cohort were found in the PCLO (33%, n=7), TP53 (29%, n=6), and LRP1B (24%, n=5) genes. Common copy number alterations included deletions in CDKN2A and CDKN2B (both 10%, n=2 each). Among the six patients with TP53 mutations, two also had concurrent deletions in CDKN2A and CDKN2B. Additionally, mutations were observed in several genes involved in chromatin organization, including CREBBP, DNMT3A, and TET2. These mutations, however, did not correlate with the therapeutic response to bortezomib and dexamethasone. There was a trend toward shorter PFS in patients with higher mutation burdens, though this did not reach statistical significance (Fig. 3A). A comparative genomic analysis before and after treatment indicated no significant changes or emergence of new mutations suggestive of disease progression. However, one patient (P14) showed an increase in mutation number in the post-treatment biopsy compared to the pre-treatment tissue, as detailed in Fig. 3B. This patient developed de novo truncating mutations in several genes including NFE2L2, CCND2, ERBB2, MET, CTNNB1, RHOA, and TGFBR2, the impact of which on disease progression remains to be further explored.

Discussion

In this study, the combination of bortezomib and dexamethasone showed considerable efficacy in treating previously treated patients with CTCLs, including advanced-stage MF, achieving an ORR of 44.8%. While earlier research indicated some efficacy of bortezomib as a single agent in CTCL patients, many experienced disease progression soon after completing the standard bortezomib regimen [23]. To address this issue, we developed a treatment approach that extends exposure to the therapeutic agent by adding a maintenance phase with a dosing schedule of once every 4 weeks, following eight cycles of induction therapy. This approach notably extended the response duration, as evidenced by improvements in both ORR and ORR4. The median PFS among responders was 14.0 months, comparable to results seen with brentuximab vedotin [11]. Importantly, the duration and quality of response are more indicative of patient survival outcomes and quality of life than merely the response rate in the treatment of CTCLs.

The extended treatment strategy may inherently lead to increased toxicities. Neurotoxicity, particularly sensory and motor peripheral neuropathy, is a significant concern with bortezomib-based treatments. However, the shift to subcutaneous administration of bortezomib has significantly lowered the incidence of neurotoxicity [24]. In a previous phase II study, our group investigated bortezomib administered weekly in combination with CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) for treating peripheral T-cell lymphomas [25]. Despite the concurrent use of vincristine, known to exacerbate neuropathy, peripheral neuropathy was less severe, prompting us to adopt this regimen in the current study. Indeed, peripheral neuropathy was reported in only 20.7% of participants, with all cases being either grade 1 or 2, which is much lower than the incidence reported in the previous phase II study using intravenous bortezomib [23]. Additionally, with weekly bortezomib administration, most hematologic toxicities were manageable.

Over the past decade, novel agents targeting surface molecules on CTCL tumor cells have been introduced and incorporated into routine care following their success in phase III studies [11,13]. Currently, other immunotherapeutic strategies, including agents targeting immune checkpoints, the killer-cell immunoglobulin-like receptor, and CAR-engineered T cells, are being explored and have shown promising results in early-stage clinical trials [14,26,27]. Despite these advancements, achieving long-term remission in CTCLs remains exceedingly rare with existing treatments [6]. Therefore, there is a pressing need to identify and validate a broader range of effective therapies. In this context, the demonstrated efficacy and safety of the bortezomib plus dexamethasone combination in the current study hold significant clinical importance. This treatment could serve as a reliable and safe element within prolonged treatment regimens for CTCLs.

In our exploratory analysis, we investigated the genomic characteristics of patients who provided tumor tissue samples for targeted sequencing. Our analysis revealed recurrent mutations in genes such as PLCO, TP53, CDKN2A, and CDKN2B among patients with relapsed or refractory CTCL. These findings are consistent with those reported in a prior study that performed targeted sequencing on 77 CTCL samples, encompassing early and advanced MF as well as other CTCL variants [28]. Notably, the prevalence of TP53 mutations in our study was 29% (n=6), which is significantly higher than the 10% to 15% mutation rates found in earlier studies [29]. Moreover, similar to observations in peripheral T-cell lymphoma (PTCL), a study reported high relapse rates in PTCL patients harboring TP53 mutations when treated with CHOP-based chemotherapy [29]. This elevated mutation frequency in our cohort may suggest a potential association between TP53 alterations and poorer outcomes following systemic cytotoxic chemotherapy.

Aligned with recent advancements in understanding the roles of genetic and epigenetic mutations, our study identified alterations in genes involved in the mitogen-activated protein kinase signaling pathway and chromatin remodeling, including BRAF, CREBBP, DNMT3A, and TET2 [28]. Despite these findings, the treatment outcomes, such as ORR and PFS, following therapy with bortezomib and dexamethasone were not directly correlated with these mutations. However, we observed a trend toward shorter PFS in patients with higher mutation loads (Fig. 3A). This suggests that mutation profiles might not consistently predict therapeutic outcomes in CTCL patients. Additionally, comparative genomic analysis performed before and after treatment did not show new mutations that could indicate disease progression, further questioning the prognostic utility of mutation profiling in CTCL. Nonetheless, the limited sample size of our study calls for further research with larger cohorts to assess the impact of genetic and epigenetic alterations more definitively.

In conclusion, the combination of bortezomib and dexamethasone has shown promising efficacy in treating relapsed and refractory CTCLs, with a durable response observed. This study supports the use of bortezomib and dexamethasone as an effective and safe treatment option for previously treated CTCLs.

Electronic Supplementary Material

Supplementary materials are available at Cancer Research and Treatment website (https://www.e-crt.org).

crt-2024-479_S1_Fig.pdf

crt-2024-479_S2_Table.xlsx

crt-2024-479_S3_Table.xlsx

crt-2024-479_S4_Table.xlsx

NOTES

Ethical Statement

This study was approved by the Institutional Review Boards of Samsung Medical Center, Korea University Anam Hospital, Seoul National University Hospital, Inje University Haeundae Paik Hospital, Jeonbuk National University Hospital, Chungnam National University Hospital, Dong-A University Hospital, Pusan National University Hospital, National Cancer Center, Asan Medical Center, Konkuk University Hospital and Ajou University Hospital. It was conducted in accordance with the ethical principles of the Declaration of Helsinki and the Korea Good Clinical Practice guidelines. All participants were enrolled this study after written informed consents.

Author Contributions

Conceived and designed the analysis: Choi YS, Shim J, Kim SJ.

Collected the data: Choi YS, Kang KW, Yoon SE, Hong JS, Lim SN, Yhim HY, Kwon JH, Lee GW, Yang DH, Oh SY, Shin HJ, Eom HS, Yoon DH, Lee HG, Jeong SH, Kim WS, Kim SJ.

Contributed data or analysis tools: Choi YS, Shim J, Kang KW, Yoon SE, Hong JS, Lim SN, Yhim HY, Kwon JH, Lee GW, Yang DH, Oh SY, Shin HJ, Eom HS, Yoon DH, Lee HG, Jeong SH, Kim WS, Kim SJ.

Performed the analysis: Choi YS, Shim J, Kim SJ.

Wrote the paper: Choi YS, Shim J, Kim SJ.

Conflicts of Interest

This study was supported by research grant and investigational products from Boryung Pharmacuetical Co. Ltd.

Acknowledgements

This work was partly supported by the National Research Foundation grants (NRF-2018M3A9D3079499 to Y.S.C. and NRF-2022R1A2-C3012346 to Y.S.C.).

Fig. 1.

CONSORT diagram and pattern of tumor response. (A) Flowchart of participants in the BIC study. (B) Swimmer plot showing duration of the study treatment and tumor responses. (C) Best change from baseline in skin by mSWAT scoring system. Bar color reflects global response according to global response criteria. AMI, acute myocardial infarction; CR, complete response; CTCL, cutaneous T-cell lymphoma; LyP, lymphomatoid papulosis; MF, mycosis fungoides; mSWAT, modified Severity-Weighted Assessment Tool; PC-ALCL, primary cutaneous anaplastic large cell lymphoma; PR, partial response; PTCL-NOS, peripheral T-cell lymphoma, unspecified; SD, stable disease.

Fig. 2.

Antitumor activity and survival outcomes of the study treatment in patients with cutaneous T-cell lymphomas. (A) Tumor response in representative advanced mycosis fungoides patients with erythrodermic and tumor stage presentations. (B) The Kaplan-Meier curves of progression-free survival (PFS) in the intention-to-treat (ITT) population (n=29). (C) The Kaplan-Meier curves of PFS in responders (n=13) and non-responders (n=16). (D) The Kaplan-Meier curves of overall survival (OS) in the ITT population. CI, confidence interval.

Fig. 3.

Landscape of somatic alterations in patients with cutaneous T-cell lymphomas (CTCLs). (A) Heatmap showing the most frequent genetic alteration in patients with relapsed or refractory CTCLs prior to initiating treatment with bortezomib and dexamethasone. Each column represents a distinct patient. (B) Longitudinal analysis of treatment-induced genomic profile changes. The heatmap illustrates the evolution of genomic profiles under the influence of bortezomib and dexamethasone treatment in patients with relapsed or refractory CTCLs. CR, complete response; LyP, lymphomatoid papulosis; MF, mycosis fungoides; MTOR, mammalian target of rapamycin; PC-ALCL, primary cutaneous anaplastic large cell lymphoma; PD, progressive disease; PFS, progression-free survival; PI3K, phosphoinositide 3-kinase; PR, partial response; PTCL-NOS, peripheral T-cell lymphoma, unspecified; SD, stable disease; Tx, therapy.

Table 1.

Baseline demographics and disease characteristics

Characteristic	Patients (n=29)
Age (yr)	59 (26-82)
Sex
Male	14 (48.3)
Female	15 (51.7)
ECOG performance status
0/1	13 (44.8)/14 (48.3)
2	2 (6.9)
Time from initial diagnosis (mo)	21 (3-193)
Histologic subtype
Mycosis fungoides/Sézary syndrome	19 (65.5)/0
Lymphomatoid papulosis	2 (6.9)
Primary cutaneous anaplastic large cell lymphoma	4 (13.8)
Peripheral T-cell lymphoma, not otherwise specified	4 (13.8)
Clinical stage at study entry
Mycosis fungoides	19 (100)
IA/IB	1 (5.3)/3 (15.8)
IIA/IIB	2 (10.5)/2 (10.5)
IIIA/IIIB	3 (15.8)/1 (5.3)
IVA/IVB	5 (26.3)/2 (10.5)
CD30⁺ primary cutaneous T-cell lymphoproliferative disorders^a)	6 (100)
T₁	2 (33.3)
T₂	3 (50.0)
T₃	1 (16.7)
Prior treatments
Ultraviolet B phototherapy	9 (31.0)
Radiation	10 (34.5)
Systemic therapy	26 (89.7)
Previous lines of systemic therapies
0-1	14 (48.3)
2-3	8 (27.6)
≥ 4	7 (24.1)
Previous systemic treatment received	26 (100)
Retinoids	2 (7.7)
Interferon-α	7 (26.9)
Cytotoxic chemotherapy	24 (92.3)
Brentuximab vedotin	5 (19.2)
Stem cell transplantation	1 (3.8)

Values are presented as median (range) or number (%). ECOG, Eastern Cooperative Oncology Group.

^a) CD30⁺ primary cutaneous T-cell lymphoproliferative disorders include lymphomatoid papulosis and primary cutaneous anaplastic large cell lymphoma.

Table 2.

Efficacy measures per ISCL-USCLC-EORTC consensus guidelines

Efficacy	No. (%) (n=29)
Best overall response
Complete response	2 (6.9)
Partial response	11 (37.9)
Stable disease	7 (24.2)
Progressive disease	9 (31.0)
Overall response rate	13 (44.8)
ORR4	10 (34.5)
Disease control rate	20 (69.0)

ORR4, an objective global response lasting (from first to last response) at least 4 months. ISCL-USCLC-EORTC, International Society for Cutaneous Lymphomas, the United States Cutaneous Lymphoma Consortium, and the European Organization for Research and Treatment of Cancer.

Table 3.

Tumor response in extracutaneous compartments

	Response in compartment
	Lymph node	Viscera	Blood
Complete response	0	0	0
Partial response	3 (10.3)	0	0
Stable disease	4 (13.8)	2 (6.9)	1 (3.4)
Progressive disease	4 (13.8)	2 (6.9)	1 (3.4)
Not involved	18 (62.1)	25 (86.2)	27 (93.2)

Values are presented as number (%).

Table 4.

Treatment-emergent adverse events

Adverse event	Grade 1-2	Grade 3	Grade 4
Hematologic events
Thrombocytopenia	2 (6.9)	0	1 (3.4)
Neutropenia	1 (3.4)	0	0
Anemia	4 (13.8)	0	0
Gastrointestinal events
Diarrhea	3 (10.3)	1 (3.4)	0
Nausea	4 (13.8)	0	0
Neurologic events
Peripheral neuropathy	6 (20.7)	0	0
Seizure	1 (3.4)	0	0
Infections
Pneumonia	0	1 (3.4)	0
Eye infection	2 (6.9)	0	0
Skin infection	0	1 (3.4)	0
Upper respiratory tract infection	3 (10.3)	0	0
Other conditions
Fatigue	5 (17.2)	0	0
Skin rash	5 (17.2)	0	0
Pyrexia	4 (13.8)	0	0
Elevated aminotransferase	2 (6.9)	0	0
Hypertension	2 (6.9)	0	0

Values are presented as number (%).

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Assessing the Efficacy of Bortezomib and Dexamethasone for Induction and Maintenance Therapy in Relapsed/Refractory Cutaneous T-Cell Lymphoma: A Phase II CISL1701/BIC Study

Cancer Res Treat. 2025;57(1):267-279. Published online July 16, 2024

DOI: https://doi.org/10.4143/crt.2024.479

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Abstract
Introduction
Materials and Methods
Results
Discussion
Electronic Supplementary Material
NOTES
REFERENCES

Assessing the Efficacy of Bortezomib and Dexamethasone for Induction and Maintenance Therapy in Relapsed/Refractory Cutaneous T-Cell Lymphoma: A Phase II CISL1701/BIC Study

Fig. 1. CONSORT diagram and pattern of tumor response. (A) Flowchart of participants in the BIC study. (B) Swimmer plot showing duration of the study treatment and tumor responses. (C) Best change from baseline in skin by mSWAT scoring system. Bar color reflects global response according to global response criteria. AMI, acute myocardial infarction; CR, complete response; CTCL, cutaneous T-cell lymphoma; LyP, lymphomatoid papulosis; MF, mycosis fungoides; mSWAT, modified Severity-Weighted Assessment Tool; PC-ALCL, primary cutaneous anaplastic large cell lymphoma; PR, partial response; PTCL-NOS, peripheral T-cell lymphoma, unspecified; SD, stable disease.

Fig. 2. Antitumor activity and survival outcomes of the study treatment in patients with cutaneous T-cell lymphomas. (A) Tumor response in representative advanced mycosis fungoides patients with erythrodermic and tumor stage presentations. (B) The Kaplan-Meier curves of progression-free survival (PFS) in the intention-to-treat (ITT) population (n=29). (C) The Kaplan-Meier curves of PFS in responders (n=13) and non-responders (n=16). (D) The Kaplan-Meier curves of overall survival (OS) in the ITT population. CI, confidence interval.

Fig. 3. Landscape of somatic alterations in patients with cutaneous T-cell lymphomas (CTCLs). (A) Heatmap showing the most frequent genetic alteration in patients with relapsed or refractory CTCLs prior to initiating treatment with bortezomib and dexamethasone. Each column represents a distinct patient. (B) Longitudinal analysis of treatment-induced genomic profile changes. The heatmap illustrates the evolution of genomic profiles under the influence of bortezomib and dexamethasone treatment in patients with relapsed or refractory CTCLs. CR, complete response; LyP, lymphomatoid papulosis; MF, mycosis fungoides; MTOR, mammalian target of rapamycin; PC-ALCL, primary cutaneous anaplastic large cell lymphoma; PD, progressive disease; PFS, progression-free survival; PI3K, phosphoinositide 3-kinase; PR, partial response; PTCL-NOS, peripheral T-cell lymphoma, unspecified; SD, stable disease; Tx, therapy.

Fig. 1.

Fig. 2.

Fig. 3.

Assessing the Efficacy of Bortezomib and Dexamethasone for Induction and Maintenance Therapy in Relapsed/Refractory Cutaneous T-Cell Lymphoma: A Phase II CISL1701/BIC Study

Characteristic	Patients (n=29)
Age (yr)	59 (26-82)
Sex
Male	14 (48.3)
Female	15 (51.7)
ECOG performance status
0/1	13 (44.8)/14 (48.3)
2	2 (6.9)
Time from initial diagnosis (mo)	21 (3-193)
Histologic subtype
Mycosis fungoides/Sézary syndrome	19 (65.5)/0
Lymphomatoid papulosis	2 (6.9)
Primary cutaneous anaplastic large cell lymphoma	4 (13.8)
Peripheral T-cell lymphoma, not otherwise specified	4 (13.8)
Clinical stage at study entry
Mycosis fungoides	19 (100)
IA/IB	1 (5.3)/3 (15.8)
IIA/IIB	2 (10.5)/2 (10.5)
IIIA/IIIB	3 (15.8)/1 (5.3)
IVA/IVB	5 (26.3)/2 (10.5)
CD30⁺ primary cutaneous T-cell lymphoproliferative disorders^a)	6 (100)
T₁	2 (33.3)
T₂	3 (50.0)
T₃	1 (16.7)
Prior treatments
Ultraviolet B phototherapy	9 (31.0)
Radiation	10 (34.5)
Systemic therapy	26 (89.7)
Previous lines of systemic therapies
0-1	14 (48.3)
2-3	8 (27.6)
≥ 4	7 (24.1)
Previous systemic treatment received	26 (100)
Retinoids	2 (7.7)
Interferon-α	7 (26.9)
Cytotoxic chemotherapy	24 (92.3)
Brentuximab vedotin	5 (19.2)
Stem cell transplantation	1 (3.8)

Efficacy	No. (%) (n=29)
Best overall response
Complete response	2 (6.9)
Partial response	11 (37.9)
Stable disease	7 (24.2)
Progressive disease	9 (31.0)
Overall response rate	13 (44.8)
ORR4	10 (34.5)
Disease control rate	20 (69.0)

	Response in compartment
	Lymph node	Viscera	Blood
Complete response	0	0	0
Partial response	3 (10.3)	0	0
Stable disease	4 (13.8)	2 (6.9)	1 (3.4)
Progressive disease	4 (13.8)	2 (6.9)	1 (3.4)
Not involved	18 (62.1)	25 (86.2)	27 (93.2)

Adverse event	Grade 1-2	Grade 3	Grade 4
Hematologic events
Thrombocytopenia	2 (6.9)	0	1 (3.4)
Neutropenia	1 (3.4)	0	0
Anemia	4 (13.8)	0	0
Gastrointestinal events
Diarrhea	3 (10.3)	1 (3.4)	0
Nausea	4 (13.8)	0	0
Neurologic events
Peripheral neuropathy	6 (20.7)	0	0
Seizure	1 (3.4)	0	0
Infections
Pneumonia	0	1 (3.4)	0
Eye infection	2 (6.9)	0	0
Skin infection	0	1 (3.4)	0
Upper respiratory tract infection	3 (10.3)	0	0
Other conditions
Fatigue	5 (17.2)	0	0
Skin rash	5 (17.2)	0	0
Pyrexia	4 (13.8)	0	0
Elevated aminotransferase	2 (6.9)	0	0
Hypertension	2 (6.9)	0	0

Table 1. Baseline demographics and disease characteristics

Values are presented as median (range) or number (%). ECOG, Eastern Cooperative Oncology Group.

CD30⁺ primary cutaneous T-cell lymphoproliferative disorders include lymphomatoid papulosis and primary cutaneous anaplastic large cell lymphoma.

Table 2. Efficacy measures per ISCL-USCLC-EORTC consensus guidelines

Table 3. Tumor response in extracutaneous compartments

Values are presented as number (%).

Table 4. Treatment-emergent adverse events

Values are presented as number (%).