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Cancer Research and Treatment > Accepted Articles
doi: https://doi.org/10.4143/crt.2020.749    [Accepted]
Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method without RNA Extraction (direct RT-LAMP) for the Detection of Lymph Node Metastasis in Early Breast Cancer
In Hee Lee1, Jin Hyang Jung2, Soo Jung Lee3, Jeeyeon Lee2, Ho Yong Park2, Ji-Young Park4, Jee Young Park4, Jae-hwan Jung5, Hyunchul Lee6, Hyo-Sung Jeon5, Yee Soo Chae3
1Division of Hematology/Oncology, Department of Internal Medicine, Daegu Catholic University Shcool of Medicine, Daegu, Korea
2Breast & Thyroid Surgery, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Korea
3Department of Oncology/Hematology, Kyungpook National Chilgok University Hospital, School of Medicine, Kyungpook National University, Kyungpook National University Cancer Research Institute, Daegu, Korea
4Department of Pathology, Kyungpook National University Chilgok Hospital, School of Medicine, Kyungpook National University, Daegu, Korea
5M Monitor, Inc., Daegu, Korea
6DNP Biotech, Inc., Daegu, Korea
Correspondence  Yee Soo Chae ,Tel: yschae@knu.ac.kr, Fax: 82-53-200-2029, Email: yschae@knu.ac.kr
Received: July 27, 2020;  Accepted: April 22, 2021.  Published online: April 26, 2021.
*In Hee Lee and Jin Hyang Jung contributed equally to this work.
ABSTRACT
Purpose
Assessing lymph node metastasis, tumor-derived DNA, or tumor-derived RNA has previously been studied in place of immunohistochemical assay. Because a direct reverse transcription loop-mediated isothermal amplification method (direct RT-LAMP) has been previously developed in order to rapidly identify viruses in place of RNA extraction, our team hypothesized that a direct RT-LAMP assay can be employed as a substitute in order to detect tumor involvement of lymph nodes within breast cancer patients.
Materials and Methods
A total amount of 92 lymph nodes removed across 40 patients possessing breast cancer were collected at Kyungpook National University Chilgok Hospital between the months of November 2015 and February 2016. All samples were then evaluated and contrasted via both a direct RT-LAMP assay and routine histopathologic examination.
Results
The sensitivity and specificity of the direct RT-LAMP assay registered at 85.7% and 100%, respectively. The positive predictive value and negative predictive value were 100% and 94.4%, respectively.
Conclusion
Direct RT-LAMP assay is capable of facilitating the detection of SLN metastasis within breast cancer patients intraoperatively possessing an excellent sensitivity via a cost-effective and time–saving manner.
Key words: Breast neoplasms, Lymphatic metastasis, Reverse transcription loop-mediated isothermal amplification
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