Effect of Platinum-Based Chemotherapy on PD-L1 Expression on Tumor Cells in Non-small Cell Lung Cancer

Junghoon Shin; Jin-Haeng Chung; Se Hyun Kim; Kyu Sang Lee; Koung Jin Suh; Ji Yun Lee; Ji-Won Kim; Jeong-Ok Lee; Jin-Won Kim; Yu-Jung Kim; Keun-Wook Lee; Jee Hyun Kim; Soo-Mee Bang; Jong-Seok Lee

doi:10.4143/crt.2018.537

Cancer Research and Treatment > Volume 51(3); 2019 > Article

Shin, Chung, Kim, Lee, Suh, Lee, Kim, Lee, Kim, Kim, Lee, Kim, Bang, and Lee: Effect of Platinum-Based Chemotherapy on PD-L1 Expression on Tumor Cells in Non-small Cell Lung Cancer

Original Article

Cancer Res Treat. 2019; 51(3): 1086-1097.

Published online: November 5, 2018

DOI: https://doi.org/10.4143/crt.2018.537

Effect of Platinum-Based Chemotherapy on PD-L1 Expression on Tumor Cells in Non-small Cell Lung Cancer

Junghoon Shin, MD¹, Jin-Haeng Chung, MD, PhD², Se Hyun Kim, MD, PhD¹, Kyu Sang Lee, MD², Koung Jin Suh, MD¹, Ji Yun Lee, MD¹, Ji-Won Kim, MD¹, Jeong-Ok Lee, MD, PhD¹, Jin-Won Kim, MD, PhD¹, Yu-Jung Kim, MD, PhD¹, Keun-Wook Lee, MD, PhD¹, Jee Hyun Kim, MD, PhD¹, Soo-Mee Bang, MD, PhD¹, Jong-Seok Lee, MD, PhD¹

¹Division of Hematology-Oncology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea

²Department of Pathology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea

Correspondence: Se Hyun Kim, MD, PhD Division of Hematology-Oncology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, 82 Gumi-ro, Bundang-gu, Seongnam 13620, Korea
Tel: 82-31-787-7071 Fax: 82-31-787-4098 E-mail: sehyunkim@snubh.org

^* Junghoon Shin and Jin-Haeng Chung contributed equally to this work.

Received September 27, 2018 Accepted November 2, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

Programmed death-1 (PD-1)/PD-1 ligand (PD-L1) axis blockades have revolutionized the treatment of advanced non-small cell lung cancer (NSCLC). We assessed the effect of platinum-based chemotherapy on tumor PD-L1 expression and its clinical implications.

Materials and Methods

We used immunohistochemistry to retrospectively evaluate the percentage of tumor cells with membranous PD-L1 staining (tumor proportion score) in paired tumor specimens obtained before and after platinum-based neoadjuvant chemotherapy (NACT) in 86 patients with NSCLC. We analyzed the correlation between the change in PD-L1 tumor proportion score and clinicopathologic characteristics, response to NACT, and survival.

Results

The PD-L1 tumor proportion score increased in a significant proportion of patients with NSCLC after platinum-based NACT (Wilcoxon signed-rank test, p=0.002). That pattern was consistent across clinically defined subgroups except for patients with partial response to NACT. Tumors from 26 patients (30.2%) were PD-L1‒negative before NACT but PD-L1-positive after NACT, whereas the reverse pattern occurred in six patients (7%) (McNemar’s test, p < 0.001). Increase in PD-L1 tumor proportion score was significantly associated with lack of response to NACT (Fisher exact test, p=0.015). There was a tendency, albeit not statistically significant, for patients with an increase in PD-L1 tumor proportion score to have shorter survival.

Conclusion

Tumor PD-L1 expression increased after platinum-based NACT in a significant proportion of patients with NSCLC. Increase in tumor PD-L1 expression may predict poor clinical outcome.

Key words: Non-small cell lung carcinoma, PD-L1, Platinum, Neoadjuvant therapy, Prognosis

Introduction

Immune checkpoint inhibitors targeting the programmed death-1 (PD-1)/PD-1 ligand (PD-L1) axis, such as pembrolizumab, nivolumab, and atezolizumab, have revolutionized the treatment of advanced non-small cell lung cancer (NSCLC). Anti‒PD-1 or anti‒PD-L1 antibodies have improved the survival of patients with untreated advanced NSCLC or with NSCLC after the failure of platinum-doublet chemotherapy [1-6]. Around 20% of previously treated patients with NSCLC respond to anti‒PD-1 or anti‒PD-L1 treatment with a median response duration of 16-20 months [3-6]. Despite long-term benefits in a subset of patients, the majority of unselected patients with NSCLC fail to respond to immune checkpoint inhibitors. PD-L1 expression on tumor cells assessed by immunohistochemistry is the most commonly used predictor of response to PD-1/PD-L1 axis inhibitors and is approved as the only companion diagnostic test for use with pembrolizumab therapy [7]. The tumor PD-L1 expression level can be dynamic over the course of treatment; however, and its predictive value is therefore limited [8-13].

Surgical resection either following (neoadjuvant) or followed by (adjuvant) platinum-based cytotoxic chemotherapy is currently the standard of care in operable, early-stage or locally advanced NSCLC [14]. Platinum agents such as cisplatin and carboplatin are the most active cytotoxic drugs used to treat NSCLC and make up an essential component of chemotherapeutic regimens both in the neoadjuvant or adjuvant setting and in the palliative setting [14]. They mainly exert antitumor activity via the formation of platinum-DNA adducts, which induces a DNA damage-recognition response and cancer cell apoptosis [15]. Preclinical evidence has provided novel insights into their mechanism of action and suggests that modulation of the immune response plays a crucial role in their tumor-killing effect [16]. Importantly, platinum-mediated immunogenic cell death involves the downregulation of both PD-L1 and PD-L2 in dendritic cells and PD-L2 in tumor cells, which results in enhanced tumor recognition by T cells [17]. Clinical trials have shown that the immunogenic effect of platinum-based therapy can be enhanced by combination with PD-1/PD-L1 axis inhibitors [2,18,19].

Few studies have investigated the optimal use of immunotherapy in patients with NSCLC who undergo surgical resection. In order to incorporate immunotherapy into the treatment of those patients, it is crucial to understand the precise impact of platinum-based neoadjuvant chemotherapy (NACT) on tumor PD-L1 expression. Little is known about the prognostic value of PD-L1 upregulation or downregulation following platinum-based chemotherapy in NSCLC. We, therefore, carried out a retrospective study of patients with NSCLC who were treated with platinum-based NACT and subsequent curative lung resection to evaluate the change in tumor PD-L1 expression patterns. We also investigated the relationships between changes in tumor PD-L1 expression upon NACT and response to chemotherapy and survival.

Materials and Methods

1. Patient eligibility and data collection

We assessed alteration of tumor PD-L1 expression following platinum-based NACT in patients fulfilling the following eligibility criteria (the landmark analysis cohort): (1) had pathologically confirmed NSCLC, (2) underwent curative lung resection after at least one cycle of platinum-based NACT between 2003 and 2014 at Seoul National University Bundang Hospital (SNUBH), and (3) had paired tumor specimens obtained before and after NACT that were adequate for PD-L1 immunostaining (Fig. 1). We analyzed the prognostic value of PD-L1 upregulation after platinum-based NACT only in the subset of patients with stage I-III disease (the survival analysis cohort). Patients with stage IV NSCLC with limited distant metastasis (e.g., solitary brain metastasis) who underwent curative surgery after platinum-based NACT were eligible for the landmark analysis cohort but not for the survival analysis cohort.

We obtained data regarding clinicopathologic characteristics, neoadjuvant treatment, and outcomes from the patients’ medical records. We determined the stage of disease according to the seventh edition of the American Joint Committee on Cancer staging guideline after reviewing the patients’ clinical records, radiographic images and reports, and pathology reports [20]. We determined dates of death either from medical records or through direct contact with the patients or their family.

2. Immunohistochemical staining for PD-L1

We performed immunostaining with antibodies targeting PD-L1 (E1L3N, 1:50, Cell Signaling Technology, Danvers, MA) on tissue slides using the Ventana BenchMark XT auto-stainer (Ventana Medical Systems, Tucson, AZ) with the ultraView Universal DAB kit (Ventana Medical Systems) according to the manufacturer’s standard recommendations. Experienced pathologists (J.H.C. and K.S.L.) determined the percentage of tumor cells with membranous PD-L1 staining of any intensity (tumor proportion score) and grouped the scores into four categories based on cutoffs used in previous studies (0, < 1%; 1, 1%-5%; 2, 5%-50%; and 3, ≥ 50%) [6,21,22].

3. Statistical analysis

We analyzed categorical variables using either chi-square tests or Fisher exact tests, as appropriate. We used Student’s t tests to compare continuous variables. We analyzed the change in the distribution of PD-L1 tumor proportion scores following NACT using the Wilcoxon signed-rank test. We assessed the change in the proportion of patients that were nominally positive for tumor PD-L1 expression by McNemar’s test, using a tumor proportion score of 1% as the cutoff for tumor PD-L1 positivity. We defined disease-free survival (DFS) as the time from surgery to recurrence or death from any cause, whichever came first. We defined overall survival (OS) as the interval from surgery to death from any cause. For estimation of DFS, we censored patients who were disease-free and alive or lost to follow-up at the time of the last assessment of disease status. For estimation of OS, we censored patients who were alive or lost to follow-up on the date of the last follow-up. We calculated DFS and OS using the Kaplan-Meier method and tested for between-group differences using log-rank tests. We tested if PD-L1 upregulation following NACT had an independent prognostic value using Cox proportional hazards regression models adjusted for combinations of patient age at the time of surgery, pretreatment clinical stage, pathologic N category, and pre-NACT or post-NACT tumor proportion scores.

All tests were two-tailed. We considered p-values < 0.05 to be statistically significant. We performed all statistical analyses using R software, ver. 3.5.0 (R Foundation for Statistical Computing, Vienna, Austria).

4. Ethical statement

The institutional review board at SNUBH reviewed and approved the study protocol (approval number: B-1612-375-301). Patient consent was waived because of the retrospective nature of the study and lack of patient interaction. All study procedures including data collection and analyses were carried out in accordance with the ethical standards of the Helsinki declaration (revised 2013; World Medical Association).

Results

1. Patient characteristics

A total of 86 patients met the criteria for the landmark analysis cohort (Fig. 1). Among those, 12 patients had stage IV NSCLC and were therefore excluded from the survival analysis cohort. The patient characteristics for each cohort are summarized in Table 1. A majority of patients were male and current or former smokers, had squamous cell carcinoma, and received at least two cycles of platinum-based NACT; only three (3.5%) and two (2.7%) patients received one cycle of NACT in the landmark analysis cohort and the survival analysis cohort, respectively. Cisplatin was used in 78 patients (90.7%) in the landmark analysis cohort and 67 patients (90.5%) in the survival analysis cohort, while the remaining patients received carboplatin in combination with paclitaxel. Neoadjuvant radiotherapy was combined either sequentially or concurrently with NACT in less than 10% of the patients in both cohorts. Approximately half of the patients in both cohorts achieved partial response (PR) to NACT according to the Response Evaluation Criteria in Solid Tumors, ver. 1.1 [23].

2. Change in tumor PD-L1 expression following platinumbased NACT

Before treatment, 41 (47.7%), 16 (18.6%), 19 (22.1%), and 10 (11.6%) patients were in the PD-L1 tumor proportion score ranges of < 1%, 1%-5%, 5%-50%, and ≥ 50%, respectively, in the landmark analysis cohort. The overall proportion of patients with positive tumor PD-L1 expression increased significantly after treatment with platinum-based NACT (45/86 [52.3%] before NACT vs. 65/86 [75.6%] after NACT; p=0.002) (Fig. 2A). That trend was consistent across subgroups stratified by sex, smoking history, histologic subtype, neoadjuvant treatment category, NACT regimen, and response to NACT (Fig. 2B-L), except for the subgroup of patients that achieved PR to NACT (Fig. 2K). The distributions of pre-NACT and post-NACT PD-L1 tumor proportion scores differed significantly within most subgroups, although they were only marginally different among males (Fig. 2B) and patients treated with taxane-platinum regimens (Fig. 2J). Notably, 26 patients were negative for tumor PD-L1 expression (tumor proportion score < 1%) before NACT and positive for tumor PD-L1 expression (tumor proportion score ≥ 1%) after NACT, whereas only six patients (7%) exhibited the reverse pattern (p < 0.001). Overall, 34 (39.5%), 12 (14%), and 40 (46.5%) patients showed an increased, decreased, or unchanged PD-L1 tumor proportion score following NACT, respectively (Fig. 3).

To assess the clinicopathologic characteristics associated with PD-L1 upregulation following NACT, we divided the patients into two groups based on the change in PD-L1 tumor proportion score following NACT. The ‘upward’ group included patients whose PD-L1 tumor proportion score increased after NACT. The ‘unchanged or downward’ group included the remaining patients. Comparison of the two groups indicated that the response to NACT was significantly correlated with the direction of change in PD-L1 tumor proportion score (p=0.015); patients who did not respond to NACT were twice as likely to show an upward change in PD-L1 tumor proportion score than those who achieved PR (Table 2). That result is in line with the results in Fig. 2K and L, which show that the proportion of tumor cells expressing PD-L1 increased after NACT only in tumors from patients who did not respond to NACT.

3. Prognostic impact of tumor PD-L1 upregulation following platinum-based NACT

During a median follow-up of 7.3 years in the survival analysis cohort, 47 patients (63.5%) experienced disease recurrence and 41 patients (55.4%) died. DFS did not differ significantly between patients with increased PD-L1 tumor proportion score following NACT and those with unchanged or decreased PD-L1 tumor proportion score following NACT (median DFS, 11.8 months vs. 12 months) (Fig. 4A). Similarly, OS also did not differ significantly between the two groups (median OS, 3.8 years vs. 7.7 years) (Fig. 4B). Those results were consistent with multivariable Cox models adjusted for predefined sets of variables (Table 3). After adjustment, PD-L1 upregulation following NACT was not significantly associated with increased or decreased DFS and OS; however, visual inspection of the survival curves and the results of the multivariable Cox models indicated a trend toward poorer OS in patients whose PD-L1 tumor proportion score increased. That tendency was consistent across subgroups defined by sex, smoking history, histologic diagnosis, pretreatment clinical stage, pathologic T category assessed at surgery, NACT regimen, and response to NACT. The hazard ratio for death was less than one only in patients who received taxane-platinum combination therapy (Fig. 5).

Discussion

We found that the PD-L1 tumor proportion score increased in a significant proportion of patients with NSCLC after treatment with platinum-based NACT. That pattern of change in tumor PD-L1 expression was consistent across clinically defined subgroups, except for the patients who achieved PR to NACT. Almost one-third of all the patients were negative for PD-L1 expression before NACT but positive for PD-L1 expression after NACT, based on a cutoff PD-L1 tumor proportion score of 1%, which is currently approved as an indication for pembrolizumab therapy after the failure of platinum-based doublet chemotherapy [3,14]. That result implies that the tumor PD-L1 expression level should be reassessed after platinum-based chemotherapy for appropriate selection of immunotherapy candidates. It also may support the design of clinical trials that incorporate PD-1/PD-L1 axis inhibitors into the treatment of patients with early-stage NSCLC.

A few retrospective studies recently investigated the alteration of tumor PD-L1 expression in patients with NSCLC who received platinum-based NACT (with or without radiotherapy) [9-13]. Those studies assessed much smaller numbers of paired tumor specimens than our study, or they focused only on a specific histologic subtype and therefore produced conflicting results. It is possible that tumor cells with PD-L1 downregulation as a result of exposure to platinum agents might undergo enhanced immune clearance, resulting in enrichment of PD-L1‒upregulated tumor cells in post-NACT tumor specimens. Indeed, our findings are consistent with the results of previous studies [8,11,13], although the small cohort size warrants confirmation in a larger cohort.

The predictive or prognostic value of tumor PD-L1 expression is an area of debate [24-28]. In our study, patients with increased PD-L1 tumor proportion score following platinum-based NACT had worse response to chemotherapy and tended to live for a shorter time than patients with unchanged or downregulated tumor PD-L1 expression, although the differences in prognosis were not statistically significant. Similarly, results from previous studies indicated that increased tumor PD-L1 expression following NACT was associated with poor response to chemotherapy and poor survival [9,10,13]. That, along with the frequent upregulation of tumor PD-L1 expression following platinum-based chemotherapy, suggests that patients with operable, early-stage or locally advanced NSCLC may benefit from postoperative PD-1/PD-L1 axis-inhibitor therapy after completion of standard neoadjuvant or adjuvant chemotherapy. The ongoing EORTC-ETOP phase 3 trial may confirm the validity of that hypothesis [29].

Our study has limitations. First and most importantly, the small numbers of patients included in the study cohorts limited the statistical power. Although we observed a consistent tendency toward shorter survival in patients with an upward change in tumor PD-L1 expression, the trend did not reach statistical significance. Additionally, the pre-NACT specimens were mostly obtained by percutaneous or transbronchial lung biopsy, whereas the post-NACT specimens were obtained by surgical resection. Therefore, there might be a systematic difference in measured tumor PD-L1 expression caused by a discrepancy in tissue acquisition techniques. Lastly, the study cohorts represented a heterogeneous patient population with various histologic subtypes and clinical stages. For that reason, most of the subgroups included only a small number of patients, which made it difficult to draw reliable conclusions from the subgroup analyses.

In summary, using matched pre-NACT and post-NACT tumor specimens obtained from patients with NSCLC, we demonstrated that the proportion of tumor cells expressing PD-L1 tended to increase after platinum-based chemotherapy, regardless of clinical characteristics and pathologic subtypes. The patients whose PD-L1 tumor proportion score increased following NACT tended to have poorer chemotherapy response and OS than patients whose PD-L1 tumor proportion score decreased or was unchanged. Our findings emphasize the need for reassessment of tumor PD-L1 expression after platinum-based chemotherapy and the potential benefit of postoperative PD-1/PD-L1 checkpoint inhibitor treatment for patients with operable NSCLC who undergo platinum-based neoadjuvant or adjuvant chemotherapy.

Conflicts of Interest

Conflict of interest relevant to this article was not reported.

Acknowledgments

This study was supported by the grants from the SNUBH Research Fund (grant number: 02-2014-042 to SHK), Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C1290 to JHC) and the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIT) (No. 2017R1A5A1015626 to JHC). This manuscript has been edited by native English-speaking experts associated with BioScience Writers LLC, Houston, TX, USA.

Fig. 1.

Flow diagram of patient selection. NSCLC, non-small cell lung cancer; NACT, neoadjuvant chemotherapy; SNUBH, Seoul National University Bundang Hospital; IHC, immunohistochemistry; PD-L1, programmed death ligand 1.

Fig. 2.

Distribution of programmed death ligand 1 (PD-L1) tumor proportion score before and after neoadjuvant chemotherapy (NACT) in the landmark analysis cohort (A). Subgroup analyses of males (B), females (C), never-smokers (D), current or former smokers (E), adenocarcinoma (F), squamous cell carcinoma (G), patients who received NACT without radiotherapy (H), gemcitabine-platinum (I), taxane-platinum (J), patients with partial response to NACT (K), and patients with stable disease (L). Numbers in bar plots indicate the number of patients included in each PD-L1 tumor proportion score category.

Fig. 3.

Representative immunohistochemical staining patterns of programmed death ligand 1 (PD-L1) in tumor specimens. (A, D) PD-L1 tumor proportion score ≥ 50% before (A) and after (D) neoadjuvant chemotherapy (NACT). (B, E) PD-L1 tumor proportion score ≥ 50% before (B) and < 1% after (E) NACT. (C, F) PD-L1 tumor proportion score < 1% before (C) and ≥ 50% after (F) NACT (A-F, ×200).

Fig. 4.

Disease-free survival (A) and overall survival (B) according to the direction of change in programmed death ligand 1 (PD-L1) tumor proportion score following platinum-based neoadjuvant chemotherapy (NACT). HR, hazard ratio; CI, confidence interval.

Fig. 5.

Subgroup analyses of the prognostic value of increased (versus unchanged or decreased) programmed death ligand 1 tumor proportion score following platinum-based neoadjuvant chemotherapy (NACT) for disease-free survival (A) and overall survival (B). Note that subgroups with less than 10 patients are omitted. The vertical dashed line indicates the hazard ratio (HR) for all patients in the survival analysis cohort. CI, confidence interval; PR, partial response; SD, stable disease.

Table 1.

Patient characteristics

Characteristic	Landmark analysis cohort (n=86)	Survival analysis cohort (n=74)
Age at surgery (yr)	62 (37-79)	62 (37-79)
Sex
Male	70 (81.4)	63 (85.1)
Female	16 (18.6)	11 (14.9)
Smoking history
Never-smoker	11 (12.8)	7 (9.5)
Current or former smoker	75 (87.2)	67 (90.5)
Histologic diagnosis
Adenocarcinoma	27 (31.4)	17 (23.0)
Squamous cell carcinoma	53 (61.6)	51 (68.9)
Others^a)	6 (7.0)	6 (8.1)
Pretreatment clinical stage
I-II	22 (25.6)	22 (29.7)
IIIA	40 (46.5)	40 (54.1)
IIIB	12 (14.0)	12 (16.2)
IV	12 (14.0)^b)	0
Pathologic T category
ypT1-2	57 (66.3)	48 (64.9)
ypT3-4	29 (33.7)	26 (35.1)
Pathologic N category
ypN0	24 (27.9)	23 (31.1)
ypN1-3	62 (72.1)	51 (68.9)
Neoadjuvant treatment category
NACT only	81 (94.2)	70 (94.6)
Chemoradiation (sequential or concurrent)	5 (5.8)	4 (5.4)
NACT regimen
Gemcitabine plus platinum	58 (67.4)	52 (70.3)
Taxane plus platinum	24 (27.9)	21 (28.4)
Others^c)	4 (4.7)	1 (1.4)
Best objective response to NACT
PR	45 (52.3)	42 (56.8)
SD	41 (47.7)	32 (43.2)

Values are presented as median (range) or number (%). NACT, neoadjuvant chemotherapy; PR, partial response; SD, stable disease.

^a) Other histologic subtypes include large cell carcinoma (n=3), adenosquamous carcinoma (n=1), pleomorphic carcinoma (n=1), and sarcomatoid carcinoma (n=1),

^b) Sites of distant metastases include brain (n=5), pleura (n=4), contralateral lung (n=2), and rib (n=1),

^c) Other NACT regimens include pemetrexed plus platinum (n=2), etoposide plus platinum (n=1), and unknown regimen (n=1) in the landmark analysis cohort, and etoposide plus platinum (n=1) in the survival analysis cohort.

Table 2.

Direction of change in PD-L1 tumor proportion score following NACT in relation to clinicopathologic characteristics

Clinicopathologic variable	Unchanged or downward (n=52)	Upward (n=34)	p-value
Age at surgery (yr)	62.5 (37-76)	61 (38-79)	0.572
Sex
Male	46 (88.5)	24 (70.6)	0.049
Female	6 (11.5)	10 (29.4)
Smoking history
Never-smoker	5 (9.6)	6 (17.6)	0.331
Current or former smoker	47 (90.4)	28 (82.4)
Histological diagnosis
Adenocarcinoma	17 (32.7)	10 (29.4)	0.476
Squamous cell carcinoma	30 (57.7)	23 (67.6)
Others	5 (9.6)	1 (2.9)
Pretreatment clinical stage
I-II	11 (21.2)	11 (32.4)	0.314
III-IV	41 (78.8)	23 (67.6)
Pathologic T category
ypT1-2	36 (69.2)	21 (61.8)	0.493
ypT3-4	16 (30.8)	13 (38.2)
Pathologic N category
ypN0	14 (26.9)	10 (29.4)	0.811
ypN1-3	38 (73.1)	24 (70.6)
NACT regimen
Gemcitabine plus platinum	36 (69.2)	22 (64.7)	0.644
Taxane plus platinum	13 (25.0)	11 (32.4)
Others	3 (5.8)	1 (2.9)
Best objective response to NACT
PR	33 (63.5)	12 (35.3)	0.015
SD	19 (36.5)	22 (64.7)

Values are presented as median (range) or number (%). PD-L1, programmed death ligand 1; NACT, neoadjuvant chemotherapy; PR, partial response; SD, stable disease.

Table 3.

Prognostic impact of PD-L1 upregulation (versus no change or downregulation) following platinum-based NACT

Model	Disease-free survival		Overall survival
Model	HR (95% CI)	p-value	HR (95% CI)	p-value
Model 1^a)	1.17 (0.67-2.03)	0.578	1.69 (0.89-3.22)	0.111
Model 2^b)	1.32 (0.73-2.39)	0.366	1.60 (0.80-3.18)	0.182
Model 3^c)	0.89 (0.46-1.70)	0.718	1.53 (0.69-3.38)	0.290

PD-L1, programmed death ligand 1; NACT, neoadjuvant chemotherapy; HR, hazard ratio; CI, confidence interval.

^a) Model 1 is adjusted for age (≥ 65 vs. < 65), pretreatment clinical stage (I-II vs. III), and pathologic N category (ypN0 vs. ypN1 vs. ypN2 vs. ypN3),

^b) Model 2 is adjusted for age (≥ 65 vs. < 65), pretreatment clinical stage (I-II vs. III), pathologic N category (ypN0 vs. ypN1 vs. ypN2 vs. ypN3), and pre-NACT PD-L1 tumor proportion score (< 1% vs. 1%-50% vs. ≥ 50%),

^c) Model 3 is adjusted for age (≥ 65 vs. < 65), pretreatment clinical stage (I-II vs. III), pathologic N category (ypN0 vs. ypN1 vs. ypN2 vs. ypN3), and post-NACT PD-L1 tumor proportion score (< 1% vs. 1-50% vs. ≥ 50%).

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