1Department of Internal Medicine, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea
2Department of Nuclear Medicine, St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea
3Department of Pathology, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Suwon, Korea
Copyright © 2015 by the Korean Cancer Association
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Variable | No. (%) |
---|---|
Median age (range, yr) | 71 (37-89) |
Gender (male:female) | 80:74 (51.9:48.1) |
Smoking | |
Never smoker | 91 (59.1) |
Former smoker | 12 (7.8) |
Current smoker | 51 (33.1) |
Stage at diagnosis | |
I | 29 (18.8) |
II | 53 (34.4) |
III | 59 (38.3) |
IV | 13 (8.4) |
Type of specimen | |
Biopsy | 78 (50.6) |
Resection | 76 (49.4) |
EGFR mutation status | |
Mutation | 62 (40.3) |
Wild type | 92 (59.7) |
Mutation type | No. (%) |
---|---|
Exon 18 | 1 (1.6) |
E709A and G719C (double) | 1 |
Exon 19 | 25 (40.3) |
E746-A750 deletion | 14 |
K745-A750 deletion | 3 |
L747-P753 deletion | 2 |
L747-A750 deletion and T751P | 1 |
L747-A750 deletion and insertion C | 1 |
L747-P753 deletion and insertion C | 1 |
Q746-T751 deletion and insertion C | 1 |
S752-I759 deletion | 1 |
T751-I759 deletion and insertion AC | 1 |
Exon 20 | 3 (4.8) |
T790M | 1 |
S768I and V769L | 1 |
Duplicate AACCCCCAC | 1 |
Exon 21 | 33 (53.2) |
L858R | 31 |
L861K | 1 |
H835L | 1 |
DNA sequencing | Anti-delE746-A750 |
Anti-L858R |
||||
---|---|---|---|---|---|---|
Positive | Negative | Total | Positive | Negative | Total | |
Exon 18 | 0 | 1 | 1 | 0 | 1 | 1 |
Exon 19 | ||||||
E746-A750 deletion | 10 | 4 | 14 | 0 | 14 | 14 |
Other deletion | 1 | 10 | 11 | 0 | 11 | 11 |
Exon 20 | 0 | 3 | 3 | 1 | 2 | 3 |
Exon 21 | ||||||
L858R | 1 | 30 | 31 | 24 | 7 | 31 |
Other | 0 | 2 | 2 | 0 | 2 | 2 |
Wild type | 1 | 91 | 92 | 2 | 90 | 92 |
Total | 13 | 141 | 154 | 27 | 127 | 154 |
No. | Immunohistochemistry | Direct DNA sequencing | Type of specimen | Histologic subtype | Staining |
|
---|---|---|---|---|---|---|
Pattern | Score | |||||
1 | Del E746-A750 ab (+) | E21 (L858R) | Resection | a | Homogeneous | 1 |
2 | Del E746-A750 ab (+) | Wild type | Biopsy | a | Homogeneous | 2 |
3 | Del E746-A750 ab (+) | E19 (K745-A750) del, E20 (Q787Q) | Resection | a | Homogeneous | 2 |
4 | Del E746-A750 ab (-) | E19 (T751-I759) del | Resection | a/p | - | 0 |
5 | Del E746-A750 ab (-) | E19 (E746-A750) del | Biopsy | a | - | 0 |
6 | Del E746-A750 ab (-) | E19 (E746-A750) del | Biopsy | a | - | 0 |
7 | Del E746-A750 ab (-) | E19 (E746-A750) del | Biopsy | a/p | - | 0 |
8 | L858R ab ⑴ | Wild type | Biopsy | a | Homogeneous | 2 |
9 | L858R ab ⑴ | E20 (T790M) | Biopsy | a/p | Homogeneous | 3 |
10 | L858R ab ⑴ | Wild type | Resection | a/p | Homogeneous | 2 |
11 | L858R ab (-) | E21 (L858R), E20 (Q787Q) | Biopsy | a | - | 0 |
12 | L858R ab (-) | E21 (L858R) | Biopsy | a | - | 0 |
13 | L858R ab (-) | E21 (L858R) | Resection | a/p/l | - | 0 |
14 | L858R ab (-) | E21 (L858R) | Resection | a | - | 0 |
15 | L858R ab (-) | E21 (L858R) | Resection | a/p/s | - | 0 |
16 | L858R ab (-) | E21 (L858R) | Resection | a/p | - | 0 |
17 | L858R ab (-) | E21 (L858R) | Biopsy | p | - | 0 |
Genotype | Sensitivity (%) | Specificity (%) | Positive predictive value (%) | Negative predictive value (%) |
---|---|---|---|---|
Biopsy/resection | ||||
E746-A750 deletion | 75/的.7 | 95.7/98.6 | 66.7/80 | 97.1/97.2 |
L858R | 80/75 | 96.8/98.3 | 85.7/92.3 | 95.3/93.7 |
E746-A750 deletion or L858R | 78.3/72.7 | 90.9/96.4 | 78.3/88.9 | 90.9/89.8 |
Overall | ||||
E746-A750 deletion | 71.4 | 97.9 | 76.9 | 97.2 |
L858R | 77.4 | 97.6 | 88.9 | 94.5 |
E746-A750 deletion or L858R | 75.6 | 94.5 | 85.0 | 90.4 |
All exon 19 mutation | 44.0 | 98.4 | 84.6 | 90.1 |
Any mutation of exon 18-21 | 58.1 | 96.7 | 92.3 | 77.4 |
Antibody | tEGFR |
p-value | ||
---|---|---|---|---|
+ | – | Total | ||
EGFR mutation | 0.284 | |||
+ | 18 | 44 | 62 | |
- | 21 | 71 | 92 | |
Total | 39 | 115 | 154 | |
DEL-specific antibody | 0.64 | |||
+ | 4 | 9 | 13 | |
- | 35 | 106 | 141 | |
Total | 39 | 115 | 154 | |
L858R-speci社c antibody | 0.125 | |||
+ | 10 | 17 | 27 | |
- | 29 | 98 | 127 | |
Total | 39 | 115 | 154 |
a, acinar; p, papillary; l, lepidic; s, solid.