Abstract

The purpose of this study was to determine the susceptibility of murine Cl300 neuroblstoma to in vivo natural killer(NK) cell mediated cytolysis and to determine if NK cells influence metastasis of this tumor. The role of NK cells in controlling the metastasis af C1300 neuroblastama to the lung was examined in A/J mice. Treatment with Interleukin-2 on days 1 through 5 after C1300 inoculation significantly decreased the tumor growth, inhibited pulmonary metastasis, and enhanced in vitro NK killing of YAC-l.Treatment with gamma Interferon on days 1 through 5 after tumuor inoculation significantly inhibited pulmonary metastasis. Antitumor effects of Interleukin-2 was stronger than that of gamma Interferon. In vivo depletion of NK cells with anti-asialo GMl significantly enhanced tumor growth and decreased host antitumor activity. These results demonstrated that in vivo elinimation of anti-asialo GM1 positive cells increased pulmonary metastasis, and in such mice, there were significant differences in metastatic potential between control and Interleukin-2, gamma Interferon treated groups.

• Keywords: Neuroblastoma, Interleukin-2, Gamma interferon, Anti-asialo GM1, Metastasis