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J Korean Cancer Assoc > Volume 28(1); 1996 > Article
Journal of the Korean Cancer Association 1996;28(1): 63-72.
암태아성항원생성 대장암세포주의 수립과 그 생물학적 성질
김진천, 이인철, 노선애, 이유경, 김경신, 박건춘
Establishment and Biological Carcterization of Human Colorectal Carcinoma Cell Lines Expressiong Carcinoembryonic Antigen
Jin Cheon Kim, In Chul Lee, Seon Ae Roh, Yoo Kyung Lee, Kyung Sin Kim, Kun Choon Park
ABSTRACT
For serum carcinoembryonic antigen (CEA) is not always elevated in recurrence or metastasis of colorectal carcinoma (CRC), activation of colorectal carcinoma cells by CEA may be selective. We have established and characterized two CEA-expressing colorectal carcinoma cell lines which were analysed in relation to the clinical course. Two cell lines originated from rectal carcinoma were cultured in DME enhanced media and subcultured by 40 and 55 times respectively. AMC 4 grew in an anchrage-independent with delayed cell doubling time (93 hrs), while AMC 5 did in an anchorage-dependent with normal doubling time (43 hrs). Tumorigenesis revealed infiltrative moderate-differentiated adenocarcinoma in AMC 4 and expansile well-differentiated adenocarcinoma in AMC 5. Karyotyping by Trypsin-Giemsa banding showed 47, XY, 21(+ ), del (2q, 5q) and 46, XY, 5(+ ), 20(+ ), 12(- ) respectively. Both cell lines were aneuploid. AMC 5 expressed large amount of 55 kD NCA as well as 180 KD CEA, but AMC 4 did traces of them by indirect immunofluorescence and immunoblot. AMC 5 cells bound optimally to solid-phase type IV collegen, laminin, and CEA by 18.7¡¾ 0.4, 21.4 ¡¾ 0.6 and 17.7¡¾0.4, while AMC 4 cells bound less. The patient of AMC 4 showed liver metastasis on 6 months after curative surgery and AMC 5 did no evidence of recurrence. In conclusion, biological characteristics of two CEA-expressing CRC cell lines seemed to be consistent with their clinical course.
Key words: Cell culture, AMC 4, AMC 5, Carcinoembryonic antigen
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