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J Korean Cancer Assoc > Volume 26(4); 1994 > Article
Journal of the Korean Cancer Association 1994;26(4): 591-599.
급성림프구성백혈병의 항원수용체유전자 재배열 양상 - Polymerase Chain Reaction ( PCR ) 을 이용한 잔존종양세포 검출을 위한 예비 연구 -
조경삼, 김영일, 김선희, 김시영, 윤휘중
Antigen Receptor Gene Rearrangement Patterans of Acute Lymphoblastic Leukemia - Preliminary study of detection of residual leukemic cells using polymerase chain r
Kyung Sam Cho, Young Il Kim, Seon Hee Kim, Si Young Kim, Hwi Joong Yoon
ABSTRACT
1) Background: Polymerase chain reaction (PCR) is a highly sensitive method to detect minimal residual disease (MRD). Although leukemia-specific translocations are detected only in minority of patients with acute lymphoblastic leukemia (ALL), several investigators developed methods to detect MRD by PCR, based on preferential usage of specific genetic elements, the limited number of VB and JB elements of T cell receptor (TCR) delta gene, and assembled V regions of immunoglobulin (Ig) gene containing re1atively conserved base sequences. 2)
Method:
We evaluated antigen receptor gene rearrangement patterns of leukemic cells from ALL patients by southern blot technique, as a baseline study for the detection of MRD using PCR. 3)
Results:
Rearrangements of Ig heavy chain, TCR beta, gamma and delta chain gene were found in 68%, 68%, 64%, 9B% of patients respectively. So thern analysis of HindIII digests with JBS16 probe showed Vδ[Jδ] rearrangement in only 4 cases. 4)
Conclusion:
Heavy chain gene rearrangement is more suitable target for the application of PCR than TCR delta gene rearrangements for detection of MRD in ALL.
Key words: Leukemia, Acute lymphoblastic, Gene rearrangement, Immunoglobulin, Gene rearrangement, T cell antigen receptor
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