Abstract

The purpose of this study was to elucidate the synergistic effect of cisplatin and 5-fluorouracil against human gastric and colon cancer cell lines. Since it is virtually impossible to document supraadditive tumor cell kill in vivo, this in vitro experiment implicates the interaction of these agents at a cellular level. We used an in vitro tetrazolium-based colorimetric assay for cytotoxicity (MTT assay) and an isobologram analysis to test combination effects of the two drugs against gastric and colon cancer cell lines, SNU-1, SNU-5, SNU-16, SNU-CI, SNU-C2A, SNU-C4, SNU-C5 those were established at cancer research institue of Seoul National University and NC1-N87 from NCI, VSA. As a resuh, those drug combination applied to this experiment didnt show a synergistic effect except in SNU-1 cell line demonstrated partly synergistic effect. Then we conclude that there are lack of in vitro synergistic effect between cisplatin and 5- fluorouracil against gastric and colon cancer cell lines, and that methodology we adopted can be one index ta check the combinatian effect of anticancer drugs.

• Keywords: Synergistic effect, Cancer cell line, MTT assay, Isobologram