| Home | E-Submission | Sitemap | Contact Us |  
J Korean Cancer Assoc > Volume 22(1); 1990 > Article
Journal of the Korean Cancer Association 1990;22(1): 37-48.
인체 암세포주의 MDR1 유전자 발현도와 항암제 감수성에 관한 연구
이경영, 박재갑, 아디가즈다, 로리골크시타인, 황이숙, 김진복
Correlation between MDR1 Gene Expression and In Vitro Drug Sensitivity Testing ( DST ) of Human Cancer Cell Lines
Kyung Yung Lee, Jae Gahb Park, Adi F. Gazdar, Lori J. Goldstein, Ee Sook Hwang, Jin Pok Kim
The MDR1 gene is frequently over-expressed in cell lines selected for multidrug resistance and certain human tumors. We correlated MDR1 gene expression with DST patterns of 6 cancer cell lines from previously untreated patients. MDR1 RNA was measured by a slot blot analysis. The concentrations of 5 drugs (etoposide, colchicine, doxorubicin, vincristine and 5-fluorouracil) that inhibited 50g of cell growth (IC50 values) were determined by the semi-automated MTT assay. Contral cell lines included KB-3-1 (sensitive) and KB-8-5 (resistant). DST indices' were calculated as follows: IC., of test cell/IC,. of KB-3-1. There was no correlation between MDR1 RNA levels and 5-fluorouracil sensitivity. Results of the other drugs (which are substrates for the MDR1 gene product); MDRI RNA DST Index Cell line Type (unit) (median) (range) KB-3-1 Control 1 1 (None) KB-8-5 Control 30 10 (7-209) SNU-1 Gastric Ca ~1 1.3 (1.0-1.4) SNUH-17 Cervix Ca ~1 3.5 (2.2-4.4) SNUH-46 Laryngeal Ca ~1 5.5 (1.8-17) SNU-47 Bone sarcoma ~1 24 (1.7-545) SNU-C4 Colorectal Ca. 30 48 (4.3-150) SNU-61 Colorectal Ca. 10 105 (2.1-440) We conclude: 1) There is no apparent relationship between MDR1 RNA levels and in vitro sensitivity to 5-fluorouracL 2) With 5 carcinoma cell lines (but not with the sarcoma line) there is an apparent relationship between MDR1 RNA levels and in vitro sensitivities to the other 4 drugs tested.
Editorial Office
Korean Cancer Association
Room 1824, Gwanghwamun Officia
92 Saemunan-ro, Jongno-gu, Seoul 03186, Korea
TEL: +82-2-3276-2410   FAX: +82-2-792-1410   E-mail: journal@cancer.or.kr
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © Korean Cancer Association.                 Developed in M2PI