Journal of the Korean Cancer Association 1989;21(1): 35-51.
Interleukin 2 에 의한 사람 종양침윤 림프구 ( TIL ) 의 활성화와 자가 고형 종야세포에 대한 항암효과
최규철, 남상윤, 박재경, 하윤문, 최용묵, 안창일
In Vitro Activation and expansion of Human Tumor - Infiltrating Lymphocytes ( TIL ) with Intreleukin 2 ( IL 2 ) and Their Antitumor - Cytotoxic Properties Against Autotogous Tumor Cells
To determine the effect of interleukin 2 (IL 2) on human tumor-infiltrating lymphocytes (TIL) from surgically excised solid tumors and to charscterize the antitwhor-cytotoxicity of activated TIL comparing with lymphokine-activated killer (LAK) cells generated from peripheral blood mononu- clear cells, this study wss performed. Single cell suspensions of TIL snd tumor cells were prepared from 20 surgical specimens (12 stomach canrers, 4 breast cancers, 1 lung cancer, 1 colon cancer, 1 ovarian cancer, and 1 cervix cancer) by mechanical and enzymatic means. And freshly isolated TIL were separated from tumor cells by fishing method. The amount of TIL obtained by the above means was 7.1x10+-6.7 (SD) cells/cm(3) of tumor mass with range of 0.8~27.5 x 10(5). The TIL prepatrations contaminated partially with tumor cells (10~20%) were cultured in IL 2 containing media. Cell numbers were reduced from the initial seeding density until about 7 days of culture. After 10 days of culture with IL 2, TIL increased in number with a concomitant disappearance of tumor cells, whereas there were slow decreases of lymphocytes and increases of tumor cells in control cultures. TIL were expanded between 0.5 and 118,000 fold increases for 10 to 34 days (2~9 subcultures). Partially purified natural IL 2-induced expansions of TIL were more rapid than highly purified recombinant IL 2-induced expansions, and showed 10 times high for 18 days of culture. The majority of TIL were T lymphocytes with range of 56~68.8% of Leu4+, 25.0~40. 8%of Leu2a+, and 0~51. 5% of Leu3a+cells. With continued in vitro expansion for 14-20 days, there was a concomitant increase in T lymphocytes (Leu4+) to 71~76.8% but the changes af phenotypes of Leu2a+ and Leu3a+ were variable. TIL were tested for cytotaxicity against fresh autologous and alfogenic tumor cells, and Raji and K562 targets in a 4-hr Cr release assay. IL 2-activated TIL showed more potent cytotoxicity against autologous tumor targets than allogenic (P<0.05). And cytotoxic activity of activated TIL in killing autologous tumor targets was higher level than that of LAK celis, but the defference was not statistically significant (0.05<p<0.1). The ability of activated TIL to kill K562 targets appeared to parallel that of LAK cells. In concluaon, TIL were induced in proliferation and augmented in their cytotoxixity by IL 2. IL 2-activated TIL preparations showed an apparent specific cytotoxicity and exhibited the combined preperties of cytotoxic T lymphocytea and LAK cells.
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