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HOME > Cancer Res Treat > Volume 46(3); 2014 > Article
Original Article Interleukin-17 Indirectly Promotes M2 Macrophage Differentiation through Stimulation of COX-2/PGE2 Pathway in the Cancer Cells
Qingli Li, MD, PhD1,2, Lunxu Liu, MD, PhD3,, Qiuyang Zhang, PhD1, Sen Liu, MD1, Dongxia Ge, MD, PhD1, Zongbing You, MD, PhD1,
Cancer Research and Treatment : Official Journal of Korean Cancer Association 2014;46(3):297-306.
DOI: https://doi.org/10.4143/crt.2014.46.3.297
Published online: July 15, 2014

1Departments of Structural and Cellular Biology and Orthopaedic Surgery, Tulane Cancer Center and Louisiana Cancer Research Consortium, Tulane Center for Stem Cell Research and Regenerative Medicine, and Tulane Center for Aging, Tulane University Health Sciences Center, New Orleans, LA, USA

2Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China

3Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China

Correspondence: Zongbing You, MD, PhD Department of Structural and Cellular Biology, Tulane University, 1430 Tulane Ave SL 49, New Orleans, LA 70112, USA 
Tel: 1-504-988-0467 Fax: 1-504-988-1687 E-mail: zyou@tulane.edu Co-Correspondence: Lunxu Liu, MD, PhD Department of Thoracic Surgery, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China 
Tel: 86-13608236600 Fax: 86-28-85422494 E-mail: lunxu_liu@aliyun.com
• Received: July 27, 2013   • Accepted: September 11, 2013

Copyright © 2014 by the Korean Cancer Association.

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/)which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Purpose
    Interleukin-17 (IL-17) is a proinflammatory cytokine that plays important roles in inflammation, autoimmunity, and cancer. The purpose of this study was to determine if IL-17 indirectly regulates macrophage differentiation through up-regulation of cyclooxygenase-2 (COX-2) expression in the cancer cell lines.
  • Materials and Methods
    Human cervical cancer HeLa, human lung cancer A549, and mouse prostate cancer Myc-CaP/CR cell lines were treated with recombinant IL-17; Western blot analysis, enzyme-linked immunosorbent assay, and quantitative real-time polymerase chain reaction analysis were utilized to examine the cellular responses.
  • Results
    IL-17 up-regulated expression of COX-2 mRNA and protein in HeLa, A549, and Myc- CaP/CR cell lines. IL-17’s effects were mediated through nuclear factor-κB and ERK1/2 signaling pathways as the inhibitors of these pathways could inhibit IL-17- induced COX-2 expression. The conditional medium obtained from the cancer cells contained prostaglandin E2, the levels of which were increased by IL-17 treatment. When treated with the conditional medium, particularly with the IL-17-induced conditional medium, mouse RAW264.7 macrophages and human THP-1 monocytes expressed higher levels of IL-10 (a marker of M2 macrophages) than inducible nitric oxide synthase or tumor necrosis factor α (markers of M1 macrophages). In contrast, when RAW264.7 and THP-1 cells were treated directly with IL-17, expression of these marker genes was not markedly changed.
  • Conclusion
    The results of this study suggest that IL-17 indirectly promotes M2 macrophage differentiation through stimulation of the COX-2/PGE2 pathway in the cancer cells, thus IL-17 plays an indirect role in regulating the tumor immune microenvironment.
  • Key words: Interleukin-17, Cyclooxygenase-2, Dinoprostone, Neoplasms, Macrophages, Tumor microenvironment
Tumor microenvironment plays an important role in tumor growth and metastasis. Tumor microenvironment consists of tumor cells and stromal cells including fibroblasts, endothelial cells, macrophages, dendritic cells, and lymphocytes, as well as these cells’ products such as extracellular matrix, cytokines, chemokines, growth factors, enzymes, and cellular metabolites. Macrophages can influence tumor growth, angiogenesis, invasion, and metastasis by expressing growth factors, cytokines, chemokines, and enzymes. The tumor-associated macrophages (TAMs) are a group of heterogeneous cells with a spectrum of diverse biological properties. The macrophages at the two ends of the spectrum are named M1 and M2 macrophages, mirroring the TH-1 and TH-2 nomenclature of T helper cells, respectively. Tumor necrosis factor α (TNFα), interferon-γ, lipopolysaccharides, and granulocyte-monocyte colony-stimulating factor are known to induce monocytes to differentiate into M1 macrophages. M1 macrophages express high levels of inducible nitric oxide synthase (iNOS), TNFα, interleukin (IL)-1β, IL-6, IL-12, IL-18, IL-23, CXC ligand 10, human leukocyte antigen-DR, and reactive oxygen and nitrogen intermediates. On the other hand, IL-4, IL-10, IL-13, IL-21, activin A, immune complexes, and glucocorticoids induce monocytes to become M2 macrophages [1]. M2 macrophages express high levels of IL-10, arginase I, IL-1 receptor antagonist, CC ligand 22, mannose receptor, galactose receptor, and CD163 antigen [1,2]. M1 macrophages inhibit tumor growth by producing effector molecules such as reactive oxygen intermediates, reactive nitrogen intermediates, and TNFα, whereas M2 macrophages promote tumor growth and metastasis by secretion of growth factors, vascular endothelial growth factor, matrix metalloproteinases, and immunosuppressive cytokines/chemokines [3]. The anti- or pro-tumor role of TAMs is determined by the balance between M1 and M2 macrophages [4]. We have previously reported that approximately 70% of TAMs are M2 macrophages and the remaining 30% are M1 macrophages in non-small cell lung cancers [5]. We have demonstrated that lung tumor tissues expressed significantly higher levels of IL-17 (also named IL-17A), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) than normal lung tissues [6]. High levels of IL-17 in the lung cancer recruit monocytes /macrophages into the lung tumor microenvironment, and PGE2 induces them to differentiate into M2 macrophages [6]. However, it is not known if IL-17 also regulates the COX-2/PGE2 pathway in the cancer cells.
IL-17 binds to a heterodimer of IL-17 receptor A (IL-17RA) and IL-17 receptor C (IL-17RC). The activated receptor complex recruits nuclear factor-κB (NF-κB) activator 1 (Act1) through SEFIR (similar expression to fibroblast growth factor genes, IL-17 receptors and Toll–IL-1R) domains that exist in IL-17RA, IL-17RC, and Act1 proteins [7]. Act1 is an E3 ubiquitin ligase that activates tumor necrosis factor receptor-associated factor 6 (TRAF6) through lysine-63-linked ubiquitination [8]. Subsequently, the polyubiquitinated TRAF6 activates transforming growth factor-β-activated kinase 1 and IκB kinase complex, resulting in activation of NF-κB pathway that induces transcription of a variety of cytokines, chemokines and growth factors [7]. In addition, IL-17 activates the extracellular signal-regulated kinases 1 and 2 (ERK1/2) that stabilizes mRNAs of the IL-17 downstream target genes [9]. In this study, we found that IL-17 activated NF-κB and ERK1/2 pathways to up-regulate expression of COX-2 mRNA and protein in HeLa, A549, and Myc-CaP/CR cancer cell lines. Subsequently, the cancer cells secreted more PGE2 that acted on monocytes to promote M2 macrophage differentiation.
1. Cell cultures
Human cervical cancer HeLa cell line, human lung cancer A549 cell line, human THP-1 monocytes (from acute monocytic leukemia), and mouse RAW264.7 macrophages (from a mouse tumor induced by Abelson murine leukemia virus) were purchased from the American Type Culture Collection (Manassas, VA). Mouse castration-resistant prostate cancer cell line Myc-CaP/CR was a gift from Dr. Leigh Ellis and Dr. Roberto Pili (Roswell Park Cancer Institute, Buffalo, NY) [10]. HeLa, A549, Myc-CaP/CR, and RAW264.7 cells were maintained in Dulbecco’s modified Eagle’s medium (Mediatech Inc., Manassas, VA) containing 10% fetal bovine serum (FBS; HyClone, Logan, UT) and 100 IU/mL penicillin/streptomycin. THP-1 cells were maintained in RPMI-1640 medium (HyClone) containing 10% FBS and 100 IU/mL penicillin/streptomycin. The cells were cultured in a 5% CO2 humidified incubator at 37°C.
2. Real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)
After 15 hours of serum starvation, HeLa, A549, and Myc-CaP/CR cells were treated without or with 20 ng/mL recombinant IL-17A (R&D Systems Inc., Minneapolis, MN) for 2hours. The cells were collected in lysis buffer and homogenized with a 1-mL syringe connected to a 21-gauge needle. Total RNA was isolated according to the instructions of RNeasy Mini Kit (Qiagen, Valencia, CA) with on-membrane DNase I digestion to avoid genomic DNA contamination. cDNA was made from total RNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). Human and mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and COX-2 PCR primers were obtained from Eurofins MWG Operon (Huntsville, AL). Real-time quantitative PCR (qRT-PCR) was performed in triplicates with an iQ5 iCycler and iQ SYBR Green Supermix (Bio-Rad Laboratories) following the recommended protocols. Results were normalized to GAPDH levels using the formula ΔCt (Cycle threshold)=Ct of target gene–Ct of GAPDH. The mRNA level of the control group was used as the baseline, so ΔΔCt was calculated using the formula ΔΔCt=ΔCt of target gene–ΔCt of the baseline. The fold change of mRNA level was calculated as fold=2-ΔΔCt.
3. Induction of monocyte/macrophage differentiation
HeLa, A549, and Myc-CaP/CR cells were cultured in serum-free medium for 15 hours and then treated without or with 20 ng/mL IL-17A for 24 hours. The culture medium was centrifuged at 14,000 ×g for 10 minutes at 4°C. The supernatant from the untreated group was named conditional medium (CM) and the supernatant from the IL-17A-treated group was named IL-17A-induced CM. Approximately 2×106 THP-1 or RAW264.7 cells were cultured in serum-free medium in 60-mm dishes for 15 hours. The CM or IL-17Ainduced CM from human HeLa or A549 cells was used to treat mouse RAW264.7 cells. Conversely, the CM or IL-17Ainduced CM from mouse Myc-CaP/CR cells was used to treat human THP-1 cells. In this way, we could avoid crosscontamination of cancer cell mRNAs in qRT-PCR analysis of macrophage mRNAs because the PCR primers were speciesspecific. In addition, the control group was treated with serum-free medium without exposure to any cells and another group was treated with IL-17A in serum-free medium to assess the direct effects of IL-17A. After 3 hours of treatment, total RNA was isolated and qRT-PCR analysis was performed as described above. Human and mouse iNOS, TNFα, IL-10, arginase I, and GAPDH PCR primers were obtained from Eurofins MWG Operon.
4. Western blot analysis
To assess the effects of IL-17 on COX-2 protein expression, 2×106 cells were cultured in serum-free medium in 60-mm dishes for 15 hours. Then, the cells were treated without or with recombinant IL-17A (20 ng/mL) for 9, 12, and 24 hours. To assess the effects of IL-17 on NF-κB and ERK1/2 pathways, 2×106 cells were cultured in serum-free medium in 60- mm dishes for 15 hours. Then, the cells were treated without or with recombinant IL-17A (20 ng/mL) for 5, 15, 60, and 180 minutes. To assess the effects of pharmacologic inhibitors on IL-17-induced COX-2 protein expression, 2×106 cells were cultured in serum-free medium in 60-mm dishes for 15 hours. Then, the cells were treated without or with 100 μM NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC; Sigma- Aldrich, St. Louis, MO) or 10 μM MEK inhibitor U0126 (Promega, Madison, WI), 30 minutes prior to addition of recombinant IL-17A (20 ng/mL) for 12 hours treatment. After the indicated time of treatment, proteins were extracted from the treated cells in RIPA lysis buffer (50 mM sodium fluoride, 0.5% Igepal CA-630 [NP-40], 10 mM sodium phosphate, 150 mM sodium chloride, 25 mM Tris pH 8.0, 1 mM phenylmethylsulfonyl fluoride, 2 mM ethylenediaminetetraacetic acid [EDTA], 1.2 mM sodium vanadate) supplemented with protease inhibitor cocktail (Sigma-Aldrich). Equal amount of proteins was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membrane. The membranes were blocked with 5% nonfat dry milk in TBST buffer (25 mM Tris-HCl, 125 mM NaCl, 0.1% Tween 20) for 2 hours and probed with the indicated primary antibodies overnight and then IRDye 800CW- or IRDye 680RD-conjugated secondary antibodies (LI-COR Biosciences, Lincoln, NE) for 1 hour. The results were visualized by using an Odyssey Infrared Imager (LICOR Biosciences). For loading control, the membranes were stripped and probed for unphosphorylated proteins and/or GAPDH. The antibodies used are as follows: mouse anti- COX-2 antibodies were obtained from Cayman Chemical (Ann Arbor, MI, USA); goat anti-COX-2 and mouse anti-PERK1/2 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); rabbit anti-ERK1/2, rabbit anti-P-IκBα, and mouse anti-IκBα antibodies were purchased from Cell Signaling Technology (Danvers, MA); mouse anti-GAPDH antibodies were ordered from Millipore Corporation (MAB374, Billerica, MA). Quantification of the Western blot signals was performed using the image analysis software of the Odyssey Infrared Imager system. The integrated density values of COX-2 signals were normalized by those of GAPDH. The ratio of COX-2/GAPDH indicates the relative level of COX-2 protein. The data represent the mean and standard deviation of three independent experiments.
5. Enzyme-linked immunosorbent assay of PGE2 levels
Approximately 2×106 cells of HeLa, A549, and Myc-CaP/CR cell lines were cultured in serum-free medium in 60-mm dishes for 15 hours. Then, the cells were treated without or with 20 ng/mL IL-17A for 24 hours. The culture medium was centrifuged at 14,000 ×g for 10 minutes at 4°C. The supernatant was collected and stored at −80°C until analysis. PGE2 levels were measured using a Prostaglandin E2 Enzyme Immunoassay Kit (Arbor Assays, Ann Arbor, MI) according to the manufacturer's instructions. Absorbance values were determined using a Bio-RAD Model 550 Microplate Reader. A standard curve was produced using a series of PGE2 concentrations from 31.25 to 1,000 pg/mL. The PGE2 level of each sample was obtained by plotting against the standard curve using CurveExpert 1.4 software (http://www.curveexpert.net). The data represent the mean and standard deviation of three independent experiments.
6. Statistical analysis
All experiments were repeated three times and the results represent mean±standard deviation of three independent experiments. Statistical analysis was made using two-tailed Student’s t-test. p < 0.05 was considered statistically significant.
1. IL-17 up-regulates expression of COX-2 mRNA and protein in cancer cell lines
We have previously shown that IL-17 up-regulates expression of chemokines and cytokines in human cancer cell lines such as HeLa, HEC-1-B, and SKOV3 [11]. In this study, we found that IL-17 significantly up-regulated COX-2 mRNA expression in HeLa, A549, and Myc-CaP/CR cancer cell lines (p < 0.05) (Fig. 1A). IL-17 also significantly up-regulated COX-2 protein expression in HeLa cells (p < 0.05) (Fig. 1B and C), A549 cells (p < 0.05) (Fig. 1D and E), and Myc-CaP/CR cells (p < 0.05) (Fig. 1F and G).
2. IL-17 activates NF-κB and/or ERK1/2 pathways in cancer cell lines
We found that IL-17A increased phosphorylated IκBα (PIκBα) after 5, 60, and 180 minutes of IL-17A treatment in HeLa cells (Fig. 2A), suggesting that NF-κB pathway was activated in the IL-17A-treated cells. In contrast, P-ERK1/2 was not increased by IL-17A treatment (Fig. 2A), suggesting that ERK1/2 pathway was not activated by IL-17A in this cancer cell line. Similarly, we found that IL-17A increased P-IκBα after 15, 60, and 180 minutes of IL-17A treatment in A549 cells, however, P-ERK1/2 was not increased except a slight increase in P-ERK2 at 15 minutes (Fig. 2B), suggesting that NF-κB pathway was also the main signaling pathway that was activated in the IL-17A-treated A549 cells while ERK1/2 signaling pathway was only slightly activated in this cell line. In contrast, we found that IL-17A increased both P-IκBα and P-ERK1/2 in Myc-CaP/CR cells (Fig. 2C), suggesting that both NF-κB and ERK1/2 signaling pathways are activated in the IL-17A-treated Myc-CaP/CR cells. To determine if NF- κB and ERK1/2 signaling pathways mediate IL-17’s effects in regulation of COX-2 expression, we treated A549 and HeLa cells with an NF-κB inhibitor PDTC or MEK inhibitor U0126 prior to IL-17A treatment. We found that U0126 dramatically and significantly inhibited IL-17A-induced COX-2 protein expression in A549 cells (p < 0.05) (Fig. 3A and C), whereas PDTC only slightly but significantly inhibited IL-17A-induced COX-2 protein expression in A549 cells (p < 0.05) (Fig. 3A and C). In HeLa cells, both U0126 and PDTC only slightly inhibited IL-17A-induced COX-2 protein expression, however, the inhibition was statistically significant (p < 0.05) (Fig. 3B and D). In Myc-CaP/CR cells, neither U0126 nor PDTC inhibited IL-17A-induced COX-2 protein expression (data not shown). These findings suggest that IL-17 indeed acts through NF-κB and ERK1/2 signaling pathways in A549 and HeLa cells to regulate expression of downstream genes, which is consistent with our previous study [11].
3. IL-17 indirectly promotes M2 macrophage differentiation through induction of PGE2 synthesis
Since COX-2 is a critical enzyme for the production of PGE2 [12], we checked if IL-17 could induce PGE2 synthesis. We found that IL-17A significantly induced synthesis and secretion of PGE2 by HeLa, A549, and Myc-CaP/CR cancer cells (p < 0.05) (Fig. 4). Since we have previously demonstrated that PGE2 can induce RAW264.7 macrophages to express marker genes of M2 macrophages such as arginase I and IL-10 [6], we investigated if IL-17A-induced CM could show similar effects. We found that IL-17A-induced CM from HeLa cells significantly increased expression of iNOS, TNFα, IL-10, and arginase I, with the highest magnitude of induction seen in IL-10 (p < 0.01 or p < 0.05, compared to the IL-17A treatment group or CM treatment group, respectively) (Fig. 5A). The CM from HeLa cells also dramatically increased expression of iNOS, TNFα, and IL-10 (Fig. 5A), representing the effects of basal levels of PGE2 that was expressed in this cell line. In contrast, directly treating RAW264.7 macrophages with IL-17A did not dramatically increase expression of iNOS, TNFα, IL-10, or arginase I (Fig. 5A). Similar findings were obtained using the CM from A549 cells, again with the highest magnitude of induction seen in IL-10 (Fig. 5B). IL-17A-induced CM from Myc-CaP/CR cells significantly increased expression of TNFα and IL-10 in THP-1 monocytes (p < 0.01, compared to the IL-17A treatment group) (Fig. 5C), and the CM from Myc-CaP/CR cells also dramatically increased expression of TNFα and IL-10, with IL-10 at a higher level than TNFα (Fig. 5C). However, there was no statistical significance between the treatments with the CM and IL-17A-induced CM (Fig. 5C). Consistently, directly treating THP-1 monocytes with IL-17A did not increase expression of iNOS, TNFα, IL-10, or arginase I (Fig. 5C).
Our previous study has demonstrated that IL-17 and PGE2 cooperatively contribute to creation of an M2-macrophagedominant tumor microenvironment in lung cancer [6]. In the present study, our data showed that IL-17 up-regulated COX-2 expression through activation of NF-κB and/or ERK1/2 signaling pathways in three cancer cell lines, namely, HeLa, A549, and Myc-CaP/CR. Subsequently, the increased COX-2 expression stimulated the synthesis and secretion of PGE2 by these cancer cells. Our findings demonstrated that the CM from these cancer cells contained PGE2. The CM induced expression of higher levels of IL-10 than iNOS or TNFα in the monocytes/macrophages, which was more obvious with the IL-17A-induced CM due to the higher levels of PGE2. It is worth pointing out that IL-17A may induce expression of other proteins besides PGE2, which may potentially affect macrophage differentiation. However, our previous study has demonstrated that PGE2 is the key heatstable factor in inducing M2 macrophage differentiation [6]. Because IL-10 is a marker of M2 macrophages [1,2], it is likely that PGE2 in the CM induced M2 macrophage differentiation as we observed previously [6]. Yet, IL-17 did not show any direct effects on the monocytes/macrophages. These findings suggest that IL-17 may indirectly affect M2 macrophage differentiation through induction of PGE2 synthesis in the cancer cells. IL-17 levels are increased in many human cancers such as lung cancer [6] and prostate cancer [13]. Recently, it was reported that TH-17 cell number and IL-17 levels are increased in cervical cancers compared to normal cervical tissues [14]. It has previously been shown that IL-17 promotes growth of human xenograft cervical tumors through recruitment of macrophages to the tumor site [15]. We have previously demonstrated that IL-17, as a chemoattractant, can directly recruit monocytes/macrophages into the tumor microenvironment [6]. In this study, we demonstrated that IL-17 can indirectly induce M2 macrophage differentiation through stimulating cancer cells to secrete PGE2. Therefore, IL-17 appears to play an important role in creation of an M2-macrophage-dominant tumor microenvironment.
IL-17 has been shown to modulate tumor immune microenvironment by recruiting myeloid-derived suppressor cells (MDSCs) and inhibiting CD8+ T cell infiltration at the tumor sites, thus promoting tumor growth [16]. Both MDSCs and M2 macrophages suppress the activity of CD8+ T cells. Therefore, our study is consistent with this previous study in showing IL-17’s role in creating an immunosuppressive tumor microenvironment. Recently, it was reported that IL-17 induces expression of granulocyte colony-stimulating factor that recruits MDSCs to the tumor microenvironment, thereby promoting tumor-resistance to anti-angiogenic therapy [17]. Another study found that blockade of IL-17 increases the cytotoxic activity of CD8+ T cells, and in contrast, eliminates MDSCs and regulatory T cells at tumor sites [18]. IL-17 has been shown to up-regulate expression of prosurvial genes, namely, myeloid cell leukemia sequence 1 (MCL1) and B-cell lymphoma 2 (Bcl-2)-related protein A1 (BCL2A1) in human dendritic cells, thus conferring chemoresistance to 11 chemotherapy agents [19]. High serum levels of IL-17 predict poor prognosis in non-small-cell lung cancer, hepatocellular carcinoma, gastric cancer, breast cancer, and colorectal cancer [20-24]. These recent findings highlight the important significance of IL-17’s function in modulating tumor immune microenvironment.
IL-17 up-regulates COX-2 expression in HeLa, A549, and Myc-CaP/CR cancer cells and subsequently increases synthesis and secretion of PGE2 that induces M2 macrophage differentiation. Therefore, IL-17 indirectly promotes M2 macrophage differentiation through stimulation of the COX-2/PGE2 pathways in cancer cells, thus IL-17 plays an indirect role in regulating the tumor immune microenvironment.

Conflict of interest relevant to this article was not reported.

Acknowledgements
The authors thank Dr. Leigh Ellis and Dr. Roberto Pili (Roswell Park Cancer Institute, Buffalo, NY) for providing the Myc-CaP/CR cell line. This work was partly supported by two grants from the National Institute of General Medical Sciences (P20GM103518) and the National Cancer Institute (R01CA174714) of the National Institutes of Health, a grant from Department of Defense (PC121647), the Developmental Fund of Tulane Cancer Center (TCC), and Louisiana Cancer Research Consortium (LCRC) Fund (to Z.Y.). The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. TCC and LCRC Core Facilities were used to conduct this study. This work was also partly supported by National Natural Science Foundation of China (NSFC 81172236: The mechanism of TAMs activation in lung cancer and a novel immunotherapy; 81372505: The role of IL- 17 in formation and progression of primary lung cancer and the underlying molecular mechanisms) and Key Science and Technology Program of Sichuan Province, China (2009SZ0152 and 2011SZ0111) (to L.L.). Dr. Qingli Li received a scholarship from the State Scholarship Fund via China Scholarship Council (CSC) for her training under Dr. Zongbing You at Tulane University.
Fig. 1.
Interleukin (IL)-17 up-regulates expression of cyclooxygenase-2 (COX-2) mRNA and protein in cancer cells. (A) The cancer cells were treated without or with 20 ng/mL IL-17A for 2 hours; COX-2 mRNA levels were determined by real-time quantitative reverse transcriptase polymerase chain reaction. (B, D, F) The cancer cells were treated with 20 ng/mL IL-17A; COX-2 protein levels were determined by Western blot analysis. (C, E, G) Quantification of Western blot signals in three independent experiments. The ratio represents COX-2 signal divided by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) signal, where ratio=1 means that COX-2 signal is equal to GAPDH signal. Values are presented as the mean± standard deviation obtained from three independent experiments. *p < 0.05, compared to the control group.
crt-46-3-297f1.gif
Fig. 2.
Interleukin (IL)-17 activates nuclear factor-κB and/or extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling pathways in cancer cells. HeLa cells (A), A549 cells (B), and Myc-CaP/CR cells (C) were treated without or with 20 ng/mL IL-17A; protein levels were determined by Western blot analysis. For protein loading control, the blots were stripped and reprobed for glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
crt-46-3-297f2.gif
Fig. 3.
U0126 and pyrrolidine dithiocarbamate (PDTC) inhibit interleukin (IL)-17-induced cyclooxygenase-2 (COX-2) protein expression in cancer cells. (A, B) The cancer cells were treated without or with 100 μM nuclear factor-κB (NF-κB) inhibitor PDTC or 10 μM MEK inhibitor U0126 for 30 minutes prior to addition of recombinant IL-17A (20 ng/mL) for 12 hours treatment. COX-2 protein expression was determined by Western blot analysis. (C, D) Quantification of Western blot signals in three independent experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05, compared to the IL-17A treatment group.
crt-46-3-297f3.gif
Fig. 4.
Interleukin (IL)-17 induces prostaglandin E2 (PGE2) secretion in cancer cells. The cancer cells were serum starved for 15 hours and then treated without or with 20 ng/mL IL-17A for 24 hours; PGE2 levels were determined using an enzyme-linked immunosorbent assay kit. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05, compared to the control group.
crt-46-3-297f4.gif
Fig. 5.
Interleukin (IL)-17A-induced conditional medium increases expression of marker genes of M2 macrophages. The conditional medium (CM) or IL-17A-induced CM from human HeLa cancer cells (A) or human A549 cancer cells (B) was used to treat mouse RAW264.7 macrophages; the CM or IL-17A-induced CM from mouse Myc-CaP/CR cancer cells (C) was used to treat human THP-1 monocytes. The control group was treated with serum-free medium that had not been exposed to any cells. The IL-17A group was treated with 20 ng/mL IL-17A in serum-free medium. After 3 hours treatment, mRNA levels of the genes were determined by real-time quantitative reverse transcriptase polymerase chain reaction. Inducible nitric oxide synthase (iNOS) and tumor necrosis factor α (TNFα) are markers for M1 macrophages, and IL-10 and arginase I are markers for M2 macrophages. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05 or **p < 0.01, compared to the groups as indicated.
crt-46-3-297f5.gif
  • 1. Mantovani A, Sica A, Locati M. New vistas on macrophage differentiation and activation. Eur J Immunol. 2007;37:14–6. ArticlePubMed
  • 2. Gordon S. Alternative activation of macrophages. Nat Rev Immunol. 2003;3:23–35. ArticlePubMed
  • 3. Mantovani A, Sica A, Locati M. Macrophage polarization comes of age. Immunity. 2005;23:344–6. ArticlePubMed
  • 4. Mantovani A, Bottazzi B, Colotta F, Sozzani S, Ruco L. The origin and function of tumor-associated macrophages. Immunol Today. 1992;13:265–70. ArticlePubMed
  • 5. Ma J, Liu L, Che G, Yu N, Dai F, You Z. The M1 form of tumorassociated macrophages in non-small cell lung cancer is positively associated with survival time. BMC Cancer. 2010;10:112.ArticlePubMedPMC
  • 6. Liu L, Ge D, Ma L, Mei J, Liu S, Zhang Q, et al. Interleukin-17 and prostaglandin E2 are involved in formation of an M2 macrophage-dominant microenvironment in lung cancer. J Thorac Oncol. 2012;7:1091–100. ArticlePubMedPMC
  • 7. Onishi RM, Gaffen SL. Interleukin-17 and its target genes: mechanisms of interleukin-17 function in disease. Immunology. 2010;129:311–21. ArticlePubMedPMC
  • 8. Liu C, Qian W, Qian Y, Giltiay NV, Lu Y, Swaidani S, et al. Act1, a U-box E3 ubiquitin ligase for IL-17 signaling. Sci Signal. 2009;2:ra63.ArticlePubMedPMC
  • 9. Hata K, Andoh A, Shimada M, Fujino S, Bamba S, Araki Y, et al. IL-17 stimulates inflammatory responses via NF-kappaB and MAP kinase pathways in human colonic myofibroblasts. Am J Physiol Gastrointest Liver Physiol. 2002;282:G1035–44. ArticlePubMed
  • 10. Ellis L, Lehet K, Ramakrishnan S, Adelaiye R, Pili R. Development of a castrate resistant transplant tumor model of prostate cancer. Prostate. 2012;72:587–91. ArticlePubMedPMC
  • 11. Lai T, Wang K, Hou Q, Zhang J, Yuan J, Yuan L, et al. Interleukin 17 induces up-regulation of chemokine and cytokine expression via activation of the nuclear factor kappaB and extracellular signal-regulated kinase 1/2 pathways in gynecologic cancer cell lines. Int J Gynecol Cancer. 2011;21:1533–9. ArticlePubMed
  • 12. Greenhough A, Smartt HJ, Moore AE, Roberts HR, Williams AC, Paraskeva C, et al. The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment. Carcinogenesis. 2009;30:377–86. ArticlePubMed
  • 13. Steiner GE, Newman ME, Paikl D, Stix U, Memaran-Dagda N, Lee C, et al. Expression and function of pro-inflammatory interleukin IL-17 and IL-17 receptor in normal, benign hyperplastic, and malignant prostate. Prostate. 2003;56:171–82. ArticlePubMed
  • 14. Chen Z, Ding J, Pang N, Du R, Meng W, Zhu Y, et al. The Th17/Treg balance and the expression of related cytokines in Uygur cervical cancer patients. Diagn Pathol. 2013;8:61.ArticlePubMedPMC
  • 15. Tartour E, Fossiez F, Joyeux I, Galinha A, Gey A, Claret E, et al. Interleukin 17, a T-cell-derived cytokine, promotes tumorigenicity of human cervical tumors in nude mice. Cancer Res. 1999;59:3698–704. PubMed
  • 16. He D, Li H, Yusuf N, Elmets CA, Li J, Mountz JD, et al. IL-17 promotes tumor development through the induction of tumor promoting microenvironments at tumor sites and myeloid-derived suppressor cells. J Immunol. 2010;184:2281–8. ArticlePubMedPMC
  • 17. Chung AS, Wu X, Zhuang G, Ngu H, Kasman I, Zhang J, et al. An interleukin-17-mediated paracrine network promotes tumor resistance to anti-angiogenic therapy. Nat Med. 2013;19:1114–23. ArticlePubMed
  • 18. Hayata K, Iwahashi M, Ojima T, Katsuda M, Iida T, Nakamori M, et al. Inhibition of IL-17A in tumor microenvironment augments cytotoxicity of tumor-infiltrating lymphocytes in tumorbearing mice. PLoS One. 2013;8:e53131ArticlePubMedPMC
  • 19. Olsson Akefeldt S, Maisse C, Belot A, Mazzorana M, Salvatore G, Bissay N, et al. Chemoresistance of human monocyte-derived dendritic cells is regulated by IL-17A. PLoS One. 2013;8:e56865ArticlePubMedPMC
  • 20. Liao R, Sun J, Wu H, Yi Y, Wang JX, He HW, et al. High expression of IL-17 and IL-17RE associate with poor prognosis of hepatocellular carcinoma. J Exp Clin Cancer Res. 2013;32:3.ArticlePubMedPMC
  • 21. Yamada Y, Saito H, Ikeguchi M. Prevalence and clinical relevance of Th17 cells in patients with gastric cancer. J Surg Res. 2012;178:685–91. ArticlePubMed
  • 22. Chen WC, Lai YH, Chen HY, Guo HR, Su IJ, Chen HH. Interleukin-17-producing cell infiltration in the breast cancer tumour microenvironment is a poor prognostic factor. Histopathology. 2013;63:225–33. ArticlePubMed
  • 23. Zhang GQ, Han F, Fang XZ, Ma XM. CD4+, IL17 and Foxp3 expression in different pTNM stages of operable non-small cell lung cancer and effects on disease prognosis. Asian Pac J Cancer Prev. 2012;13:3955–60. ArticlePubMed
  • 24. Liu J, Duan Y, Cheng X, Chen X, Xie W, Long H, et al. IL-17 is associated with poor prognosis and promotes angiogenesis via stimulating VEGF production of cancer cells in colorectal carcinoma. Biochem Biophys Res Commun. 2011;407:348–54. ArticlePubMed

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REFERENCES

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    Citations to this article as recorded by  
    • Innate immune cells: Key players of orchestra in modulating tumor microenvironment (TME)
      Mahvash Sadeghi, Sajad Dehnavi, Moosa Sharifat, Amir Mohammad Amiri, Ali Khodadadi
      Heliyon.2024; 10(5): e27480.     CrossRef
    • Phase II trial of pembrolizumab, ipilimumab, and aspirin in melanoma: clinical outcomes and translational predictors of response
      Zoe Quandt, Saya Jacob, Muhammad Zaki Hidayatullah Fadlullah, Chaorong Wu, Clinton Wu, Laura Huppert, Lauren S. Levine, Paula Sison, Katy K. Tsai, Melissa Chow, Jee Hye Kang, Jimmy Hwang, James C. Lee, Ariel Oglesby, Jessica Venegas, Ben J. Brintz, Aik Ch
      BJC Reports.2024;[Epub]     CrossRef
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      Jingyuan Li, Jun Tan, Tao Wang, Shan Yu, Guangliang Guo, Kan Li, Le Yang, Bin Zeng, Xueying Mei, Siyong Gao, Xiaomei Lao, Sien Zhang, Guiqing Liao, Yujie Liang
      Experimental Hematology & Oncology.2024;[Epub]     CrossRef
    • Breast cancer colonization by Malassezia globosa accelerates tumor growth
      Miao-Miao Liu, Hui-Hui Zhu, Jie Bai, Zi-Ye Tian, Yu-Jing Zhao, Teun Boekhout, Qi-Ming Wang, Jianping Xu
      mBio.2024;[Epub]     CrossRef
    • The cytokine profile correlates with less tumor-infiltrating lymphocytes in luminal A breast cancer
      Eri Ishikawa, Takahiro Watanabe, Takako Kihara, Mamiko Kuroiwa, Miki Komatsu, Sayaka Urano, Masayuki Nagahashi, Seiichi Hirota, Yasuo Miyoshi
      Breast Cancer Research and Treatment.2024;[Epub]     CrossRef
    • Anti-PD-1 antibody-activated Th17 cells subvert re-invigoration of antitumor cytotoxic T-lymphocytes via myeloid cell-derived COX-2/PGE2
      Qingsheng Li, Kevin E. Goggin, SeonYeong Seo, Jonathan M. Warawa, Nejat K. Egilmez
      Cancer Immunology, Immunotherapy.2023; 72(4): 1047.     CrossRef
    • Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis
      Xu Wang, Shuangshaung Wu, Wenjie Wu, Wenqing Zhang, Linman Li, Qian Liu, Zhimin Yan, Jianping Xu
      mBio.2023;[Epub]     CrossRef
    • Tumor Necrosis Factor-α Promotes the Tumorigenesis, Lymphangiogenesis, and Lymphatic Metastasis in Cervical Cancer via Activating VEGFC-Mediated AKT and ERK Pathways
      Xiao Chen, Luping Lin, Qiaoling Wu, Sang Li, Huihui Wang, Yang Sun, Jinghua Pan
      Mediators of Inflammation.2023; 2023: 1.     CrossRef
    • Depletion of enhancer zeste homolog 2 (EZH2) directs transcription factors associated with T cell differentiation through epigenetic regulation of Yin Yang 1(YY1) in combating non-small cell lung cancer (NSCLC)
      Nikhil Chandnani, Vedika Shrirang Choudhari, Rajat Talukdar, Sudeshna Rakshit, Geetha Shanmugam, Shiuli Guchait, Ishika Gupta, Melvin George, Koustav Sarkar
      Medical Oncology.2023;[Epub]     CrossRef
    • The role of IL-17 in lung cancer growth
      Liping Liu, Renli Liu, Chaojie Wei, Dong Li, Xiuzhu Gao
      Cytokine.2023; 169: 156265.     CrossRef
    • IL-17A functions and the therapeutic use of IL-17A and IL-17RA targeted antibodies for cancer treatment
      Meiying Song, Jie Liang, Luoyang Wang, Wei Li, Suli Jiang, Shuo Xu, Lei Tang, Qiaochu Du, Guixian Liu, Haining Meng, Dongchang Zhai, Shangheng Shi, Yanyan Yang, Li Zhang, Bei Zhang
      International Immunopharmacology.2023; 123: 110757.     CrossRef
    • Effect of perioperative acupoint electrical stimulation on macrophages in mice under operative stress
      Yinzhou Zhang, Junying Wei, Xinyuan Wu, Mengting Jiang, Wuhua Ma, Yuhui Li
      Journal of Inflammation.2023;[Epub]     CrossRef
    • Obesity-Modified CD4+ T-Cells Promote an Epithelial-Mesenchymal Transition Phenotype in Prostate Cancer Cells
      Alejandra De Angulo, Peyton Travis, Gloria Cecilia Galvan, Christopher Jolly, Linda deGraffenried
      Nutrition and Cancer.2022; 74(2): 650.     CrossRef
    • Tumor immunotherapy: Mechanisms and clinical applications
      Cheng Nong, Pengbo Guan, Li Li, Huiyuan Zhang, Hongbo Hu
      MedComm – Oncology.2022;[Epub]     CrossRef
    • Interleukin-17D promotes lung cancer progression by inducing tumor-associated macrophage infiltration via the p38 MAPK signaling pathway
      Zhenzhen Lin, Qiumin Huang, Junrong Liu, Hao Wang, Xuexi Zhang, Zhiyan Zhu, Wei Zhang, Yiliang Wei, Zhe Liu, Wei Du
      Aging.2022; 14(15): 6149.     CrossRef
    • Prostate Infiltration by Treg and Th17 Cells as an Immune Response to Propionibacterium acnes Infection in the Course of Benign Prostatic Hyperplasia and Prostate Cancer
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      International Journal of Molecular Sciences.2022; 23(16): 8849.     CrossRef
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      Cell Communication and Signaling.2022;[Epub]     CrossRef
    • Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
      Janine Trombke, Lucie Loyal, Julian Braun, Uwe Pleyer, Andreas Thiel, Dominika Pohlmann
      Scientific Reports.2021;[Epub]     CrossRef
    • MicroRNA-146a limits tumorigenic inflammation in colorectal cancer
      Lucien P. Garo, Amrendra K. Ajay, Mai Fujiwara, Galina Gabriely, Radhika Raheja, Chantal Kuhn, Brendan Kenyon, Nathaniel Skillin, Ryoko Kadowaki-Saga, Shrishti Saxena, Gopal Murugaiyan
      Nature Communications.2021;[Epub]     CrossRef
    • Molecular insights into phytochemicals‐driven break function in tumor microenvironment
      Priyanshi Rana, Amarjeet Shrama, Chandi C. Mandal
      Journal of Food Biochemistry.2021;[Epub]     CrossRef
    • Assessment of the presence of interleukin 17+ macrophages and Th17 cells in situ in lip and oral tongue cancer
      Thâmara Manoela Marinho Bezerra, Bárbara Vanessa de Brito Monteiro, Joabe dos Santos Pereira, Luiz Arthur Barbosa Silva, Cassiano Francisco Weege Nonaka, Éricka Janine Dantas da Silveira, Márcia Cristina da Costa Miguel
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    • Dysregulated tumor-associated macrophages in carcinogenesis, progression and targeted therapy of gynecological and breast cancers
      Tianhan Xu, Sihui Yu, Jiawen Zhang, Sufang Wu
      Journal of Hematology & Oncology.2021;[Epub]     CrossRef
    • Danger matrix molecules orchestrate CD14/CD44 signaling in cancer development
      Heiko Roedig, Roxana Damiescu, Jinyang Zeng-Brouwers, Iva Kutija, Jonel Trebicka, Malgorzata Wygrecka, Liliana Schaefer
      Seminars in Cancer Biology.2020; 62: 31.     CrossRef
    • IL-17A Modulates Peritoneal Macrophage Recruitment and M2 Polarization in Endometriosis
      Jessica E. Miller, Soo Hyun Ahn, Ryan M. Marks, Stephany P. Monsanto, Asgerally T. Fazleabas, Madhuri Koti, Chandrakant Tayade
      Frontiers in Immunology.2020;[Epub]     CrossRef
    • Interferon-Gamma Stimulated Murine MacrophagesIn Vitro: Impact of Ionic Composition and Osmolarity and Therapeutic Implications
      Joshua Erndt-Marino, Daniel J. Yeisley, Hongyu Chen, Michael Levin, David L. Kaplan, Mariah S. Hahn
      Bioelectricity.2020; 2(1): 48.     CrossRef
    • Pleiotropic Role and Bidirectional Immunomodulation of Innate Lymphoid Cells in Cancer
      Zhengwen An, Fabian Flores-Borja, Sheeba Irshad, Jinhai Deng, Tony Ng
      Frontiers in Immunology.2020;[Epub]     CrossRef
    • CD4+ T helper 17 cell response of aged mice promotes prostate cancer cell migration and invasion
      Sen Liu, Fengli Liu, Bing Zhang, Peng Yan, Brian G. Rowan, Asim B. Abdel‐Mageed, Chad Steele, S. Michal Jazwinski, Krzysztof Moroz, Elizabeth B. Norton, Alun Wang, Leann Myers, Oliver Sartor, Qiuyang Zhang
      The Prostate.2020; 80(10): 764.     CrossRef
    • Concanavalin-A stimulates IL-17 and nitric oxide production and induces macrophage polarization and resistance to Trypanosoma cruzi infection
      Nágela Ghabdan Zanluqui, Maria Isabel Lovo-Martins, Aparecida Donizette Malvezi, Carolina Panis, Rosiane Valeriano da Silva, Vera Lucia Hideko Tatakihara, Ionice Felipe, Marli Cardoso Martins-Pinge, Pryscilla Fanini Wowk, Phileno Pinge-Filho
      Life Sciences.2020; 258: 118137.     CrossRef
    • ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages
      Jinlong Yin, Sung Soo Kim, Eunji Choi, Young Taek Oh, Weiwei Lin, Tae-Hoon Kim, Jason K. Sa, Jun Hee Hong, Se Hwan Park, Hyung Joon Kwon, Xiong Jin, Yeonhee You, Ji Hye Kim, Hyunggee Kim, Jaekyoung Son, Jeongwu Lee, Do-Hyun Nam, Kui Son Choi, Bingyang Shi
      Nature Communications.2020;[Epub]     CrossRef
    • miR-708-5p targets oncogenic prostaglandin E2 production to suppress a pro-tumorigenic phenotype in lung cancer cells
      Nicholas J. Monteleone, Carol S. Lutz
      Oncotarget.2020; 11(26): 2464.     CrossRef
    • Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3 Pathway
      Bowei Zhang, Yingchuan Xu, Shuang Liu, Huan Lv, Yaozhong Hu, Yaya Wang, Zhi Li, Jin Wang, Xuemeng Ji, Hui Ma, Xiaowen Wang, Shuo Wang
      Nutrients.2020; 12(8): 2367.     CrossRef
    • Cellular and Molecular Progression of Prostate Cancer: Models for Basic and Preclinical Research
      Sirin Saranyutanon, Sachin Kumar Deshmukh, Santanu Dasgupta, Sachin Pai, Seema Singh, Ajay Pratap Singh
      Cancers.2020; 12(9): 2651.     CrossRef
    • Innate Lymphoid Cells in the Malignant Melanoma Microenvironment
      Aintzane Apraiz, Aitor Benedicto, Joana Marquez, Andrea Agüera-Lorente, Aintzane Asumendi, Elvira Olaso, Beatriz Arteta
      Cancers.2020; 12(11): 3177.     CrossRef
    • Role of interleukin-17 in acute myocardial infarction
      Mónica Daniela Mora-Ruíz, Francisco Blanco-Favela, Adriana Karina Chávez Rueda, María Victoria Legorreta-Haquet, Luis Chávez-Sánchez
      Molecular Immunology.2019; 107: 71.     CrossRef
    • The Formation and Therapeutic Update of Tumor-Associated Macrophages in Cervical Cancer
      Qun Wang, Alexander Steger, Sven Mahner, Udo Jeschke, Helene Heidegger
      International Journal of Molecular Sciences.2019; 20(13): 3310.     CrossRef
    • IL-17A improves the efficacy of mesenchymal stem cells in ischemic-reperfusion renal injury by increasing Treg percentages by the COX-2/PGE2 pathway
      Ming Bai, Li Zhang, Bo Fu, Jiuxu Bai, Yingjie Zhang, Guangyan Cai, Xueyuan Bai, Zhe Feng, Shiren Sun, Xiangmei Chen
      Kidney International.2018; 93(4): 814.     CrossRef
    • Mucosa-associated invariant T cells in malignancies: a faithful friend or formidable foe?
      S. M. Mansour Haeryfar, Christopher R. Shaler, Patrick T. Rudak
      Cancer Immunology, Immunotherapy.2018; 67(12): 1885.     CrossRef
    • Contribution to Tumor Angiogenesis From Innate Immune Cells Within the Tumor Microenvironment: Implications for Immunotherapy
      Adriana Albini, Antonino Bruno, Douglas M. Noonan, Lorenzo Mortara
      Frontiers in Immunology.2018;[Epub]     CrossRef
    • Prostaglandin E2 Secreted by Thyroid Cancer Cells Contributes to Immune Escape Through the Suppression of Natural Killer (NK) Cell Cytotoxicity and NK Cell Differentiation
      Arum Park, Yunhee Lee, Mi Sun Kim, Young Ju Kang, Young-Jun Park, Haiyoung Jung, Tae-Don Kim, Hee Gu Lee, Inpyo Choi, Suk Ran Yoon
      Frontiers in Immunology.2018;[Epub]     CrossRef
    • β-Catenin Regulation in Sporadic Colorectal Carcinogenesis: Not as Simple as APC
      Ernst Fredericks, Gill Dealtry, Saartjie Roux
      Canadian Journal of Gastroenterology and Hepatology.2018; 2018: 1.     CrossRef
    • The promotion of tissue engineering blood vessel patency by CGS21680 through regulating pro‐inflammatory activities of endothelial progenitor cell
      Wen Chen, Li Xiao, Jian Bai, Wen Zeng, Mingcan Yang, Bingyi Shi, Chuhong Zhu
      Journal of Biomedical Materials Research Part A.2018; 106(10): 2634.     CrossRef
    • ST2/IL-33 signaling promotes malignant development of experimental squamous cell carcinoma by decreasing NK cells cytotoxicity and modulating the intratumoral cell infiltrate
      Nádia Ghinelli Amôr, Carine Ervolino de Oliveira, Thaís Helena Gasparoto, Vanessa Garcia Vilas Boas, Graziela Perri, Ramon Kaneno, Vanessa Soares Lara, Gustavo Pompermaier Garlet, João Santana da Silva, Gislâine A. Martins, Cory Hogaboam, Karen A. Cavassa
      Oncotarget.2018; 9(56): 30894.     CrossRef
    • Interleukin-17 acts as double-edged sword in anti-tumor immunity and tumorigenesis
      Xin Qian, Hankui Chen, Xiaofeng Wu, Ling Hu, Qi Huang, Yang Jin
      Cytokine.2017; 89: 34.     CrossRef
    • Metformin inhibits castration-induced EMT in prostate cancer by repressing COX2/PGE2/STAT3 axis
      Dali Tong, Qiuli Liu, Gaolei Liu, Jing Xu, Weihua Lan, Yao Jiang, Hualiang Xiao, Dianzheng Zhang, Jun Jiang
      Cancer Letters.2017; 389: 23.     CrossRef
    • Inflammation-Related Pancreatic Carcinogenesis
      Juan-juan Dai, Ming-jie Jiang, Xing-Peng Wang, Ling Tian
      Pancreas.2017; 46(8): 973.     CrossRef
    • The paradox of Th17 cell functions in tumor immunity
      Zahra Asadzadeh, Hamed Mohammadi, Elham Safarzadeh, Maryam Hemmatzadeh, Ahmad Mahdian-shakib, Farhad Jadidi-Niaragh, Gholamreza Azizi, Behzad Baradaran
      Cellular Immunology.2017; 322: 15.     CrossRef
    • IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection
      Jaleesa M. Garth, Kristen M. Reeder, Matthew S. Godwin, Joseph J. Mackel, Chad W. Dunaway, Jonathan P. Blackburn, Chad Steele
      The Journal of Immunology.2017; 199(6): 2140.     CrossRef
    • Apolipoprotein A-I mimetic peptide 4F suppresses tumor-associated macrophages and pancreatic cancer progression
      Meiyu Peng, Qi Zhang, Yingnan Cheng, Shuyu Fu, Huipeng Yang, Xiangdong Guo, Jieyou Zhang, Lina Wang, Lijuan Zhang, Zhenyi Xue, Yan Li, Yurong Da, Zhi Yao, Liang Qiao, Rongxin Zhang
      Oncotarget.2017; 8(59): 99693.     CrossRef
    • Myeloid-derived cells in prostate cancer progression: phenotype and prospective therapies
      Zoila Lopez-Bujanda, Charles G Drake
      Journal of Leukocyte Biology.2017; 102(2): 393.     CrossRef
    • hnRNPA2/B1 activates cyclooxygenase‐2 and promotes tumor growth in human lung cancers
      Yang Xuan, Jingshu Wang, Liying Ban, Jian-Jun Lu, Canhui Yi, Zhenglin Li, Wendan Yu, Mei Li, Tingting Xu, Wenjing Yang, Zhipeng Tang, Ranran Tang, Xiangsheng Xiao, Songshu Meng, Yiming Chen, Quentin Liu, Wenlin Huang, Wei Guo, Xiaonan Cui, Wuguo Deng
      Molecular Oncology.2016; 10(4): 610.     CrossRef
    • Culture supernatants of cervical cancer cells induce an M2 phenotypic profile in THP-1 macrophages
      Eliza Julia Pedraza-Brindis, Karina Sánchez-Reyes, Georgina Hernández-Flores, Alejandro Bravo-Cuellar, Luis Felipe Jave-Suárez, Adriana Aguilar-Lemarroy, Paulina Gómez-Lomelí, Brenda Anahí López-López, Pablo Cesar Ortiz-Lazareno
      Cellular Immunology.2016; 310: 42.     CrossRef
    • 15-Lipoxygenase metabolites of α-linolenic acid, [13-(S)-HPOTrE and 13-(S)-HOTrE], mediate anti-inflammatory effects by inactivating NLRP3 inflammasome
      Naresh Kumar, Geetika Gupta, Kotha Anilkumar, Naireen Fatima, Roy Karnati, Gorla Venkateswara Reddy, Priyanka Voori Giri, Pallu Reddanna
      Scientific Reports.2016;[Epub]     CrossRef
    • Crosstalk between Innate Lymphoid Cells and Other Immune Cells in the Tumor Microenvironment
      Fabian Flores-Borja, Sheeba Irshad, Peter Gordon, Felix Wong, Ibrahim Sheriff, Andrew Tutt, Tony Ng
      Journal of Immunology Research.2016; 2016: 1.     CrossRef
    • Phytomedicine—Modulating oxidative stress and the tumor microenvironment for cancer therapy
      Yu-Ting Cheng, Chun-Chih Yang, Lie-Fen Shyur
      Pharmacological Research.2016; 114: 128.     CrossRef
    • The Role of Interleukin-17 in Lung Cancer
      Feng Wu, Juanjuan Xu, Qi Huang, Jieli Han, Limin Duan, Jinshuo Fan, Zhilei Lv, Mengfei Guo, Guorong Hu, Lian Chen, Shuai Zhang, Xiaonan Tao, Wanli Ma, Yang Jin
      Mediators of Inflammation.2016; 2016: 1.     CrossRef
    • Calreticulin Promotes Proliferation and Migration But Inhibits Apoptosis in Schwann Cells
      Gui Huang, Zhulei Sun, Jiang Wu, Shaofeng Shui, Xinwei Han, Dong Guo, Tengfei Li
      Medical Science Monitor.2016; 22: 4516.     CrossRef
    • The immunopathogenesis of birdshot chorioretinopathy; a bird of many feathers
      Jonas Kuiper, Aniki Rothova, Joke de Boer, Timothy Radstake
      Progress in Retinal and Eye Research.2015; 44: 99.     CrossRef
    • Genetic basis of hypersensitivity reactions to nonsteroidal anti-inflammatory drugs
      Francisca Gómez, James R. Perkins, Elena García-Martín, Gabriela Canto, José A. Cornejo-García
      Current Opinion in Allergy & Clinical Immunology.2015; 15(4): 285.     CrossRef
    • CD4, IL-17, and COX-2 Are Associated With Subclinical Inflammation in Malar Melasma
      Adriana Rodríguez-Arámbula, Bertha Torres-Álvarez, Diego Cortés-García, Cornelia Fuentes-Ahumada, Juan Pablo Castanedo-Cázares
      The American Journal of Dermatopathology.2015; 37(10): 761.     CrossRef
    • Cyclooxygenase-2 promotes tumor growth and suppresses tumor immunity
      Bing Liu, Liyan Qu, Shigui Yan
      Cancer Cell International.2015;[Epub]     CrossRef
    • M2 macrophage accumulation in the aortic wall during angiotensin II infusion in mice is associated with fibrosis, elastin loss, and elevated blood pressure
      Jeffrey P. Moore, Antony Vinh, Kellie L. Tuck, Samy Sakkal, Shalini M. Krishnan, Christopher T. Chan, Maggie Lieu, Chrishan S. Samuel, Henry Diep, Barbara K. Kemp-Harper, Marianne Tare, Sharon D. Ricardo, Tomasz J. Guzik, Christopher G. Sobey, Grant R. Dr
      American Journal of Physiology-Heart and Circulatory Physiology.2015; 309(5): H906.     CrossRef
    • Macrophage depletion reduces postsurgical tumor recurrence and metastatic growth in a spontaneous murine model of melanoma
      Muly Tham, Karen Khoo, Kim Pin Yeo, Masashi Kato, Amelle Prevost-Blondel, Veronique Angeli, Jean-Pierre Abastado
      Oncotarget.2015; 6(26): 22857.     CrossRef
    • Interleukin-17A Promotes Arginase-1 Production and 2,4-Dinitrochlorobenzene-Induced Acute Hyperinflammation in Human Papillomavirus E7 Oncoprotein-Expressing Skin
      Le Son Tran, Deepak Mittal, Stephen R. Mattarollo, Ian H. Frazer
      Journal of Innate Immunity.2015; 7(4): 392.     CrossRef

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      Interleukin-17 Indirectly Promotes M2 Macrophage Differentiation through Stimulation of COX-2/PGE2 Pathway in the Cancer Cells
      Cancer Res Treat. 2014;46(3):297-306.   Published online July 15, 2014
      DOI: https://doi.org/10.4143/crt.2014.46.3.297
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    Interleukin-17 Indirectly Promotes M2 Macrophage Differentiation through Stimulation of COX-2/PGE2 Pathway in the Cancer Cells
    Image Image Image Image Image
    Fig. 1. Interleukin (IL)-17 up-regulates expression of cyclooxygenase-2 (COX-2) mRNA and protein in cancer cells. (A) The cancer cells were treated without or with 20 ng/mL IL-17A for 2 hours; COX-2 mRNA levels were determined by real-time quantitative reverse transcriptase polymerase chain reaction. (B, D, F) The cancer cells were treated with 20 ng/mL IL-17A; COX-2 protein levels were determined by Western blot analysis. (C, E, G) Quantification of Western blot signals in three independent experiments. The ratio represents COX-2 signal divided by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) signal, where ratio=1 means that COX-2 signal is equal to GAPDH signal. Values are presented as the mean± standard deviation obtained from three independent experiments. *p < 0.05, compared to the control group.
    Fig. 2. Interleukin (IL)-17 activates nuclear factor-κB and/or extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling pathways in cancer cells. HeLa cells (A), A549 cells (B), and Myc-CaP/CR cells (C) were treated without or with 20 ng/mL IL-17A; protein levels were determined by Western blot analysis. For protein loading control, the blots were stripped and reprobed for glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
    Fig. 3. U0126 and pyrrolidine dithiocarbamate (PDTC) inhibit interleukin (IL)-17-induced cyclooxygenase-2 (COX-2) protein expression in cancer cells. (A, B) The cancer cells were treated without or with 100 μM nuclear factor-κB (NF-κB) inhibitor PDTC or 10 μM MEK inhibitor U0126 for 30 minutes prior to addition of recombinant IL-17A (20 ng/mL) for 12 hours treatment. COX-2 protein expression was determined by Western blot analysis. (C, D) Quantification of Western blot signals in three independent experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05, compared to the IL-17A treatment group.
    Fig. 4. Interleukin (IL)-17 induces prostaglandin E2 (PGE2) secretion in cancer cells. The cancer cells were serum starved for 15 hours and then treated without or with 20 ng/mL IL-17A for 24 hours; PGE2 levels were determined using an enzyme-linked immunosorbent assay kit. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05, compared to the control group.
    Fig. 5. Interleukin (IL)-17A-induced conditional medium increases expression of marker genes of M2 macrophages. The conditional medium (CM) or IL-17A-induced CM from human HeLa cancer cells (A) or human A549 cancer cells (B) was used to treat mouse RAW264.7 macrophages; the CM or IL-17A-induced CM from mouse Myc-CaP/CR cancer cells (C) was used to treat human THP-1 monocytes. The control group was treated with serum-free medium that had not been exposed to any cells. The IL-17A group was treated with 20 ng/mL IL-17A in serum-free medium. After 3 hours treatment, mRNA levels of the genes were determined by real-time quantitative reverse transcriptase polymerase chain reaction. Inducible nitric oxide synthase (iNOS) and tumor necrosis factor α (TNFα) are markers for M1 macrophages, and IL-10 and arginase I are markers for M2 macrophages. Values are presented as the mean±standard deviation obtained from three independent experiments. *p < 0.05 or **p < 0.01, compared to the groups as indicated.

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    Interleukin-17 Indirectly Promotes M2 Macrophage Differentiation through Stimulation of COX-2/PGE2 Pathway in the Cancer Cells
    Cancer Res Treat : Cancer Research and Treatment
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