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J Korean Cancer Assoc > Volume 30(1); 1998 > Article
Journal of the Korean Cancer Association 1998;30(1): 12-19.
p53 Mutation of Head and Neck Squamous Cell Carcinoma Cell Lines
Chung Hwan Baek, Ye Jeung Ko, Young Ik Sun, Sung Wha Hong, Kwang Chol Chu
Department of Otorhinolaryngology, College of Medicine, Sung Kyun Kwan University, Samsung Medical Center.
Structural alterations of p53 and overexpression of the p53 protein are the most common genetic abnormalities in various kinds of human cancers. In this study, we examined the mutational status and the frequency of p53 mutations in head and neck squamous cell carcimona (HNSCC) cell lines.
7 human head and neck squamous cell carcinoma cell lines were included in this analysis. Using polymerase chain reaction(PCR), single strand confonmation polymorphism(SSCP) and PCR-DNA sequencing analysis, we tested the mutational status of 7 cell lines. Exon 4~9 of the p53 gene was amplified for the direct DNA sequencing analysis.
Our results showed 100% nuclear p53 immunostaining and 3 electrophoretic abnomalities by PCR-SSCP in three cancer cell lines and mutations of the p53 gene including 2 base substitutions and 1 base deletion were detected in 3 cancer cell lines using PCR directed DNA sequencing analysis.
7 HNSCC cell lines examined in this study provide excellent systems for study of gene therapy using p53 gene.
Key words: p53 mutations;HNSCC;PCR-SSCP;Sequencing
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