KCA
Search
- Page Path
- HOME > Search
Original Article
- Telomerase Activity and Expression of hTR and TERT in Human Soft Tissue Sarcomas
- Jinyoung Yoo, Seok Jin Kang, Byung Kee Kim
- Cancer Res Treat. 2002;34(1):46-51. Published online February 28, 2002
- DOI: https://doi.org/10.4143/crt.2002.34.1.46
-
Abstract
PDF - PURPOSE
Sarcomas have rarely been analyzed for telomerase, which is an RNA-dependent DNA polymerase to maintain telomeres and prevent telomere shortening. This study was undertaken to determine telomerase activity and the expression of the telomerase subunits human telomerase RNA (hTR) and telomerase reverse transcriptase (TERT) in soft tissue sarcomas.
MATERIALS AND METHODS
Twenty three sarcomas were analyzed for the telomerase activity by a radioactive PCR-based TRAP assay. All of the samples were further investigated for the expression of hTR by in situ hybridization and for TERT and p53 by immunohistochemistry.
RESULTS
Telomerase activity was detected in four (17%) samples. Expression of hTR was demonstrated in 11 (48%) cases, whereas TERT was expressed in 20 (87%).Of the four telomerase-positive tumors, three were positive for both hTR and TERT, and one was positive only for TERT. p53 overexpression was observed in nine (39%) tumors. The frequency of p53 expression increased as the tumor grade advanced (p= .064).
CONCLUSION
These data indicate that the reactivation of telomerase is an uncommon event in human soft tissue sarcomas. The high frequency of the expression of hTR and TERT in these tumors suggests that telomerase activity may be regulated at the transcriptional level and an additional event leading to telomerase activation exist. -
Citations
Citations to this article as recorded by
- Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer
Hui Yang, Hongyan Zhang, Yahua Zhong, Qiaoli Wang, Lei Yang, Hong Kang, Xiaojia Gao, Haijun Yu, Conghua Xie, Fuxiang Zhou, Yunfeng Zhou
Scientific Reports.2017;[Epub] CrossRef
- Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer
- 4,359 View
- 28 Download
- 1 Crossref
First
Prev
