Cancer Research and Treatment

Original Articles

Postoperative Radiotherapy Alone Versus Chemoradiotherapy in Stage I-II Endometrial Carcinoma: An Investigational and Propensity Score Matching Analysis: Jong Hoon Lee, Hyo Chun Lee, Sung Hwan Kim, Mi Joo Chung, Song Mi Jeong, Sung Jong Lee, Joo Hee Yoon, Dong Choon Park; Cancer Res Treat. 2015;47(2):298-305. Published online September 15, 2014; DOI: https://doi.org/10.4143/crt.2014.038

Purpose
The purpose of this study was to compare the results of postoperative adjuvant radiotherapy (RT) and concurrent chemoradiotherapy (CRT) in stage I-II endometrial carcinoma. Materials and Methods We analyzed a total of 64 patients with surgically staged I-II endometrial carcinoma who were treated with postoperative adjuvant RT or concurrent CRT between March 1999 and July 2013. Thirty-two patients who received postoperative RT alone were matched with those who received postoperative CRT (n=32) in accordance to age, stage, and tumor histology. Overall survival and relapse-free survival, as well as toxicity of the RT and CRT arms were evaluated and compared. Results The 5-year overall survival rate was 90.0% for the RT arm and 91.6% for the CRT arm. There was no significant difference in overall survival between the two treatment arms (p=0.798). The 5-year relapse-free survival rate was 87.2% in the RT arm and 88.0% in the CRT arm. Again, no significant difference in relapse-free survival was seen between the two arms (p=0.913). In a multivariate analysis, tumor histology was an independent prognostic factor for relapse-free survival (hazard ratio, 3.67; 95% of CI, 2.34 to 7.65; p=0.045). Acute grade 3 or 4 hematologic toxicities in the CRT arm were significantly higher than in the RT alone arm (6.2% vs. 31.2%, p=0.010). Conclusion Adjuvant pelvic concurrent chemoradioherapy did not show superior results in overall survival and relapse-free survival compared to RT alone in stage I-II endometrial carcinoma.

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Impacts of Postoperative Adjuvant Therapies on the Survival of Women with High-Risk Early-Stage Endometrial Cancer: A Cohort Study
Hee Joong Lee, Banghyun Lee
Cancers.2025; 17(2): 187. CrossRef
Adjuvant Therapy Use and Survival in Stage II Endometrial Cancer
Nataniel H. Lester-Coll, Melissa R. Young, Henry S. Park, Elena S. Ratner, Babak Litkouhi, Shari Damast
International Journal of Gynecological Cancer.2017; 27(9): 1904. CrossRef

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cDNA Microarray Analysis of Gene Expression Profiles Associated with Cervical Cancer: Joo Hee Yoon, Joon Mo Lee, Sung Eun Namkoong, Su Mi Bae, Yong Wan Kim, Sei Jun Han, Young Lae Cho, Gye Hyun Nam, Chong Kook Kim, Jeong Sun Seo, Woong Shick Ahn; Cancer Res Treat. 2003;35(5):451-459. Published online October 31, 2003; DOI: https://doi.org/10.4143/crt.2003.35.5.451

Abstract PDF

PURPOSE
The molecular pathology of cervical cancers associated with human papillomavirus infection is presently unclear. In an effort to clarify this issue, the gene expression profiles and pathogenic cellular processes of cervical cancer lesions were investigated. MATERIALS AND METHODS: Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, The Catholic University of Korea. The disease status was assigned according to the International Federation of Gynecology and Obstetrics. The tissue samples of 11 patients (invasive cancer stage Ib- IIIa) were investigated by a cDNA microarray of 4, 700 genes, hierarchical clustering and the Gene Ontology (GO). Total RNA from cervical cancer and non-lesional tissues were labeled with Cy5 and Cy3. The HaCaT human epithelial keratinocyte cell line was used as a negative control cell. The stages of invasive cancer were Ib to IIIb. All specimens were obtained by punch-biopsies and frozen in liquid nitrogen until required. RESULTS: 74 genes, showing more than a 2 fold difference in their expressions, were identified in at least 8 of the 11 patients. Of these genes, 33 were up-regulated and 41 were down-regulated. The gene expression profiles were classified into 345 mutually dependent function sets, resulting in 611 cellular processes according to their GO. The GO analysis showed that cervical carcinogenesis underwent complete down-regulation of cell death, protein biosynthesis and nucleic acid metabolism. The genes related to nucleic acid binding and structural molecule activity were also significantly down-regulated. In contrast, significant up-regulation was shown in the skeletal development, immune response and extracellular activity. CONCLUSION: These data are suggestive of potentially significant pathogenetic cellular processes, and showed that the down-regulated functional profiling has an important impact on the discovery of pathogenic pathways in cervical carcinogenesis. GO analysis can also overcome the complexity of the expression profiles of the cDNA microarray via a cellular process level approach. Thereby, a valuable prognostic candidate gene, with real relevance to disease-specific pathogenesis, can be found at the cellular process levels.

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The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia
Przemysław Podstawski, Marcin Samiec, Maria Skrzyszowska, Tomasz Szmatoła, Ewelina Semik-Gurgul, Katarzyna Ropka-Molik
International Journal of Molecular Sciences.2022; 23(4): 1970. CrossRef
Cervical cancer heterogeneity: a constant battle against viruses and drugs
Qian Sun, Liangliang Wang, Cong Zhang, Zhenya Hong, Zhiqiang Han
Biomarker Research.2022;[Epub] CrossRef
Identification of the possible therapeutic targets in the insulin-like growth factor 1 receptor pathway in a cohort of Egyptian hepatocellular carcinoma complicating chronic hepatitis C type 4
Nada M.K. Mabrouk, Dalal M. Elkaffash, Mona Abdel-Hadi, Salah-ElDin Abdelmoneim, Sameh Saad ElDeen, Gihan Gewaifel, Khaled A. Elella, Maher Osman, Nahed Baddour
Drug Target Insights.2020; 14(1): 1. CrossRef
Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples
E. Semik, T. Ząbek, A. Gurgul, A. Fornal, T. Szmatoła, K. Pawlina, M. Wnuk, J. Klukowska‐Rötzler, C. Koch, K. Mählmann, M. Bugno‐Poniewierska
Veterinary and Comparative Oncology.2018; 16(1): 37. CrossRef
Transcriptome analysis of equine sarcoids
E. Semik, A. Gurgul, T. Ząbek, K. Ropka‐Molik, C. Koch, K. Mählmann, M. Bugno‐Poniewierska
Veterinary and Comparative Oncology.2017; 15(4): 1370. CrossRef
INPP5E Preserves Genomic Stability through Regulation of Mitosis
Elizabeth A. Sierra Potchanant, Donna Cerabona, Zahi Abdul Sater, Ying He, Zejin Sun, Jeff Gehlhausen, Grzegorz Nalepa
Molecular and Cellular Biology.2017;[Epub] CrossRef
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis
Yoshinori Asano, Tatsuya Kawase, Atsushi Okabe, Shuichi Tsutsumi, Hitoshi Ichikawa, Satoko Tatebe, Issay Kitabayashi, Fumio Tashiro, Hideo Namiki, Tadashi Kondo, Kentaro Semba, Hiroyuki Aburatani, Yoichi Taya, Hitoshi Nakagama, Rieko Ohki
Scientific Reports.2016;[Epub] CrossRef
Hypoxic regulation of MYBL1, MEST, TCF3, TCF8, GTF2B, GTF2F2 and SNAI2 genes expression in U87 glioma cells upon IRE1 inhibition
O. H. Minchenko, D. O. Tsymbal, D. O. Minchenko, O. O. Kubaychuk
The Ukrainian Biochemical Journal.2016; 88(6): 52. CrossRef
Phosphatidylinositolphosphate phosphatase activities and cancer
Simon A. Rudge, Michael J.O. Wakelam
Journal of Lipid Research.2016; 57(2): 176. CrossRef
Gene expression profiling in the human keratinocyte cell line, hacat exposed to urushiol isolated from sap of korean lacquer tree (Rhus verniciflua Stokes)
Ju Youn Park, Jae Ho Chang, Myong Jo Kim, Ju Sung Kim, Soo-Ki Kim
Molecular & Cellular Toxicology.2010; 6(1): 79. CrossRef
Phosphoinositide signalling in cancer: beyond PI3K and PTEN
Tom D. Bunney, Matilda Katan
Nature Reviews Cancer.2010; 10(5): 342. CrossRef
The role of the inositol polyphosphate 5-phosphatases in cellular function and human disease
Lisa M. Ooms, Kristy A. Horan, Parvin Rahman, Gillian Seaton, Rajendra Gurung, Dharini S. Kethesparan, Christina A. Mitchell
Biochemical Journal.2009; 419(1): 29. CrossRef
Altered MicroRNA Expression in Cervical Carcinomas
Jeong-Won Lee, Chel Hun Choi, Jung-Joo Choi, Young-Ae Park, Seung-Jun Kim, Seung Yong Hwang, Woo Young Kim, Tae-Joong Kim, Je-Ho Lee, Byoung-Gie Kim, Duk-Soo Bae
Clinical Cancer Research.2008; 14(9): 2535. CrossRef
Identification of hemoglobin‐α and ‐β subunits as potential serum biomarkers for the diagnosis and prognosis of ovarian cancer
Ahn Woong‐Shick, Park Sung‐Pil, Bae Su‐Mi, Lee Joon‐Mo, Namkoong Sung‐Eun, Nam Gye‐Hyun, Cho Young‐Lae, Choi Ho‐Sun, Jun Heung‐Jae, Kim Chong‐Kook, Kim Young‐Wan, Han Byoung‐Don, Jin Hyun‐Sun
Cancer Science.2005; 96(3): 197. CrossRef
The Differential Gene Expression Profiles between Sensitive and Resistant Breast Cancer Cells to Adriamycin by cDNA Microarray
Myung-Ju Ahn, Ki-Hwan Lee, Joon-Ik Ahn, Dong-Hyun Yu, Hye-Sook Lee, Jung-Hye Choi, Joung Soon Jang, Jong Min Bae, Yong-Sung Lee
Cancer Research and Treatment.2004; 36(1): 43. CrossRef

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New Approaches to Functional Process Discovery in HPV 16-Associated Cervical Cancer Cells by Gene Ontology: Yong Wan Kim, Min Je Suh, Jin Sik Bae, Su Mi Bae, Joo Hee Yoon, Soo Young Hur, Jae Hoon Kim, Duck Young Ro, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn; Cancer Res Treat. 2003;35(4):304-313. Published online August 31, 2003; DOI: https://doi.org/10.4143/crt.2003.35.4.304

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BAG3 down-modulation sensitizes HPV18+ HeLa cells to PEITC-induced apoptosis and restores p53
Roberta Cotugno, Anna Basile, Elena Romano, Dario Gallotta, Maria Antonietta Belisario
Cancer Letters.2014; 354(2): 263. CrossRef
Expression Profiling of the Cellular Processes in Uterine Leiomyomas: Omic Approaches and IGF-2 Association with Leiomyosarcomas
Su Mi Bae, Yong-Wan Kim, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn
Cancer Research and Treatment.2004; 36(1): 31. CrossRef

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Cell-Specific Growth Inhibition of Human Cervical Cancer Cell by Recombinant Adenovirus p53 in vitro and in vivo: Su Mi Bae, Yong Wook Kim, Joo Hee Yoon, Jin Young Yoo, Young Seok Seo, Sang Lyun Nam, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Yong Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2003;35(3):181-190. Published online June 30, 2003; DOI: https://doi.org/10.4143/crt.2003.35.3.181

Abstract PDF

PURPOSE
Despite the significance of the p53 adenoviral vector in cancer gene therapy, an advanced strategy for the development of preferential tumor cell-specific delivery and the long-term persistent gene expression control of p53 are required. In this study, the time-course expression patterns of p53 and E6, on cervical cancer cells, were investigated to obtain a molecular level understanding of the cell-dependent tumor growth suppression effects of a recombinant adenovirus expressing p53, both in vitro and in vivo. MATERIALS AND METHODS: The expressions of p53 and E6 in CaSki, SiHa, HeLa, HeLaS3, C33A and HT3 cervical cancer cell lines were examined. After infection with AdCMVp53, the cell growth inhibition was studied via cell count, MTT and Neutral red assays. After transfecting the AdCMVp53 and AdCMVLacZ into the cancer cells-xenografted nude mice, the anti-tumor effects were investigated for one month. RESULTS: The p53 protein levels were more notably expressed in the CaSki and HeLa than in the SiHa and HeLaS3 On day 6, the p53 was only detected in the HeLaS3. In contrast, the p53 expression was highly maintained in the C33A and HT3. The E6 mRNA levels gradually decreased in only the CaSki and HeLa. The growth suppression effects also showed cell-dependent patterns, which were consistent with the reciprocal expression patterns of p53 and E6. After transfection of the AdCMVp53, into the CaSki- and SiHa-xenografted nude mice, the tumor size was remarkably decreased in the SiHa cells as compared to that in the AdCMVLacZ transfected mice, indicating cell-specific growth inhibition patterns.
CONCLUSION
The adenovirus-mediated p53 gene transfection was very effective both in vitro and in vivo. Also, the anti-tumor effects were accomplished via the differential role of p53-specific apoptotic cell death, which was dependent on the cervical cancer cell line.

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Immunization with Adenoviral Vectors Carrying Recombinant IL-12 and E7 Enhanced the Antitumor Immunity against Human Papillomavirus 16-associated Tumor
Eun-Kyung Park, Young-Wook Kim, Joon-Mo Lee, Sung-Eun NamKoong, Do-Gang Kim, Heung-Jae Chun, Byoung-Don Han, Su-Mi Bae, Hyun-Sun Jin, Jeong-Im Sin, Woong-Shick Ahn
Cancer Research and Treatment.2005; 37(1): 63. CrossRef

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