Cancer Research and Treatment

Original Articles

Inter-alpha Inhibitor H4 as a Potential Biomarker Predicting the Treatment Outcomes in Patients with Hepatocellular Carcinoma: Eun-Jung Lee, Seung-Hyun Yang, Kyoung-Jin Kim, Hyejung Cha, Seo Jin Lee, Ji-Hye Kim, Junkyu Song, Kyung-Hee Chun, Jinsil Seong; Cancer Res Treat. 2018;50(3):646-657. Published online July 17, 2017; DOI: https://doi.org/10.4143/crt.2016.550

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Purpose
Early prediction of treatment outcomes represents an essential step towards increased treatment efficacy and survival in patients with hepatocellular carcinoma (HCC). In this study, we performed two-dimensional electrophoresis (2-DE) followed by protein profiling to identify biomarkers predictive of therapeutic outcomes in patients with HCC who received liver-directed therapy (LDTx) involving local radiotherapy (RT), and studied the underlying mechanisms of the identified proteins.
Materials and Methods
2-DE analysis was conducted by pooling sera from patients with a good or poor prognosis; serum proteomic profiles of the two groups were compared and analyzed using matrixassisted laser desorption/ionization time-of-flight mass spectrometry. Identified proteins were confirmed via enzyme-linked immunosorbent assay. An invasion assay was performed after overexpression and knockdown of target protein in Huh7 cells.
Results
Levels of inter-alpha inhibitor H4 (ITIH4), fibrinogen gamma chain, keratin 9/1 complex, carbonic anhydrase I, and carbonmonoxyhemoglobin S were changed by more than 4-fold in response to LDTx. In particular, pre-LDTx ITIH4 expression was more than 5-fold higher in patients with a good prognosis, compared to patients with a poor prognosis. The migration ability of Huh7 cells was significantly suppressed and enhanced by ITIH4 overexpression and knockdown, respectively. The tumors of patients with HCC and a good prognosis expressed high levels of ITIH4, compared to those of patients with a poor prognosis.
Conclusion
Taken together, ITIH4 may be a potential therapeutic target that could inhibit cancer metastasis, as well as a prognostic marker for patients with HCC who are receiving LDTx.

Citations

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Mass spectrometry-based proteomics of cerebrospinal fluid in pediatric central nervous system malignancies: a systematic review with meta-analysis of individual patient data
Christian Mirian, Maria Thastrup, René Mathiasen, Kjeld Schmiegelow, Jesper Velgaard Olsen, Ole Østergaard
Fluids and Barriers of the CNS.2024;[Epub] CrossRef
Inter-alpha-trypsin inhibitor heavy chain H3 is a potential biomarker for disease activity in myasthenia gravis
Christina B. Schroeter, Christopher Nelke, Frauke Stascheit, Niklas Huntemann, Corinna Preusse, Vera Dobelmann, Lukas Theissen, Marc Pawlitzki, Saskia Räuber, Alice Willison, Anna Vogelsang, Adela Della Marina, Hans-Peter Hartung, Nico Melzer, Felix F. Ko
Acta Neuropathologica.2024;[Epub] CrossRef
The function of the inter-alpha-trypsin inhibitors in the development of disease
Xin-feng Zhang, Xiao-li Zhang, Li Guo, Yun-ping Bai, Yan Tian, Hua-you Luo
Frontiers in Medicine.2024;[Epub] CrossRef
Gene Expression Profiling of Pancreatic Ductal Adenocarcinoma Arising From Intraductal Papillary Mucinous Neoplasms of the Pancreas
William A. Ziaziaris, Christopher S. H. Lim, Loretta Sioson, Anthony J. Gill, Jaswinder S. Samra, Sumit Sahni, Anubhav Mittal
Cancer Medicine.2024;[Epub] CrossRef
Characterization of the Limbal Epithelial Stem Cell Niche
Isabel Y. Moreno, Arian Parsaie, Tarsis F. Gesteira, Vivien J. Coulson-Thomas
Investigative Opthalmology & Visual Science.2023; 64(13): 48. CrossRef
Leveraging Blood-Based Diagnostics to Predict Tumor Biology and Extend the Application and Personalization of Radiotherapy in Liver Cancers
Franziska Hauth, Hannah J. Roberts, Theodore S. Hong, Dan G. Duda
International Journal of Molecular Sciences.2022; 23(4): 1926. CrossRef
Regulatory network identified by pulmonary transcriptome and proteome profiling reveals extensive change of tumor-related genes in microRNA-21 knockout mice
Ge Luan, Ming Wang, Jing Yuan, Xiangting Bu, Jing Song, Chengshuo Wang, Luo Zhang
Journal of Cancer Research and Clinical Oncology.2022; 148(8): 1919. CrossRef
Identification of main influencing factors on the protein corona composition of PLGA and PLA nanoparticles
Hendrik Spreen, Matthias Behrens, Dennis Mulac, Hans-Ulrich Humpf, Klaus Langer
European Journal of Pharmaceutics and Biopharmaceutics.2021; 163: 212. CrossRef
Clinical Assay for the Early Detection of Colorectal Cancer Using Mass Spectrometric Wheat Germ Agglutinin Multiple Reaction Monitoring
I-Jung Tsai, Emily Chia-Yu Su, I-Lin Tsai, Ching-Yu Lin
Cancers.2021; 13(9): 2190. CrossRef
Liver-specific over-expression of Cripto-1 in transgenic mice promotes hepatocyte proliferation and deregulated expression of hepatocarcinogenesis-related genes and signaling pathways
Yu Liu, Yan-Qing Li, Shi-Hao Huang, Yong-Long Li, Jia-Wei Xia, Jun-Shuang Jia, Fang Wei, Jia-Hong Wang, Guan-Qi Dai, Yu-Cai Wang, Xiao-Yan Li, Liu-Xin Han, Xiao-Ling Zhang, Xu-Dong Xiang, Wen-Tao Zhao, Dong Xiao, Xiao-Lin Lin
Aging.2021; 13(17): 21155. CrossRef
ITIH4 is a novel serum biomarker for early gastric cancer diagnosis
Yingying Sun, Jie Jin, Hongyan Jing, Yingying Lu, Qingqing Zhu, Changjuan Shu, Qinghua Zhang, Dadao Jing
Clinica Chimica Acta.2021; 523: 365. CrossRef
The Alterations in Methylene Blue/Light-Treated Frozen Plasma Proteins Revealed by Proteomics
Tiange Wu, Xiaoning Wang, Kai Ren, Xiaochen Huang, Jiankai Liu
Transfusion Medicine and Hemotherapy.2021; 48(5): 298. CrossRef
The use of minimally invasive biomarkers for the diagnosis and prognosis of hepatocellular carcinoma
Lucas Trevisan França de Lima, Daniel Broszczak, Xi Zhang, Kim Bridle, Darrell Crawford, Chamindie Punyadeera
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer.2020; 1874(2): 188451. CrossRef
Improved survival prognostication of node-positive malignant melanoma patients utilizing shotgun proteomics guided by histopathological characterization and genomic data
Lazaro Hiram Betancourt, Krzysztof Pawłowski, Jonatan Eriksson, A. Marcell Szasz, Shamik Mitra, Indira Pla, Charlotte Welinder, Henrik Ekedahl, Per Broberg, Roger Appelqvist, Maria Yakovleva, Yutaka Sugihara, Kenichi Miharada, Christian Ingvar, Lotta Lund
Scientific Reports.2019;[Epub] CrossRef
Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer
Xiao Jiang, Xiao-Yan Bai, Bowen Li, Yanan Li, Kangkai Xia, Miao Wang, Shujing Li, Huijian Wu
Disease Markers.2019; 2019: 1. CrossRef

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Overexpression of SOX2 Is Associated with Better Overall Survival in Squamous Cell Lung Cancer Patients Treated with Adjuvant Radiotherapy: Hong In Yoon, Kyu Hyun Park, Eun-Jung Lee, Ki Chang Keum, Chang Geol Lee, Chul Hoon Kim, Yong Bae Kim; Cancer Res Treat. 2016;48(2):473-482. Published online August 12, 2015; DOI: https://doi.org/10.4143/crt.2015.116

Abstract PDF PubReader ePub

Purpose
The purpose of this study is to investigate the prognostic significance of SOX2 gene amplification and expression in patients with American Joint Committee on Cancer stage III lung squamous cell carcinoma (SCC) who underwent surgery followed by adjuvant radiotherapy.
Materials and Methods
Pathological specimens were obtained from 33 patients with stage III lung SCC treated with surgery followed by adjuvant radiotherapy between 1996 and 2008. SOX2 gene amplification and protein expression were analyzed using fluorescent in situ hybridization and immunohistochemistry, respectively. Patients were divided into two groups according to their SOX2 gene amplification and protein expression status. Kaplan-Meier estimates and a Cox proportional hazards model were used to identify the prognostic factors affecting patient survival.
Results
The median follow-up period for surviving patientswas 58 months (range, 5 to 102 months). SOX2 gene amplification was observed in 22 patients and protein overexpression in 26 patients. SOX2 overexpression showed significant association with SOX2 gene amplification (p=0.002). In multivariate analysis, SOX2 overexpression was a significant prognostic factor for overall survival (OS) (hazard ratios [HR], 0.1; 95% confidence interval [CI], 0.002 to 0.5; p=0.005) and disease-free survival (DFS) (HR, 0.15; 95% CI, 0.04 to 0.65; p=0.01). Age (HR, 0.33; 95% CI, 0.11 to 0.98; p=0.046) and total radiation dose (HR, 0.13; 95% CI, 0.02 to 0.7; p=0.02) were the independent prognostic factors for OS and DFS. Patients with SOX2 amplification did not show a longer OS (p=0.95) and DFS (p=0.48).
Conclusion
Our data suggested that SOX2 overexpression could be used as a positive prognostic factor in patients with stage III lung SCC receiving adjuvant radiotherapy.

Citations

Citations to this article as recorded by

Identifying the key hub genes linked with lung squamous cell carcinoma by examining the differentially expressed and survival genes
Anushka Pravin Chawhan, Norine Dsouza
Molecular Genetics and Genomics.2024;[Epub] CrossRef
Gene‐level dissection of chromosome 3q locus amplification in squamous cell carcinoma of the lung using the nCounter assay
Taesung Jeon, Uk Jeen Oh, Jaeyoung Min, Chungyeul Kim
Thoracic Cancer.2023; 14(26): 2635. CrossRef
The role of SOX family members in solid tumours and metastasis
Daniela Grimm, Johann Bauer, Petra Wise, Marcus Krüger, Ulf Simonsen, Markus Wehland, Manfred Infanger, Thomas J. Corydon
Seminars in Cancer Biology.2020; 67: 122. CrossRef
Blood-Based SOX2-Promoter Methylation in Relation to Exercise and PM2.5 Exposure among Taiwanese Adults
Chun-Lang Su, Disline Manli Tantoh, Ying-Hsiang Chou, Lee Wang, Chien-Chang Ho, Pei-Hsin Chen, Kuan-Jung Lee, Oswald Ndi Nfor, Shu-Yi Hsu, Wen-Miin Liang, Yung-Po Liaw
Cancers.2020; 12(2): 504. CrossRef
Epithelial-Mesenchymal Transition in Skin Cancers: A Review
Anastasia Hodorogea, Andreea Calinescu, Mihaela Antohe, Mihaela Balaban, Roxana Ioana Nedelcu, Gabriela Turcu, Daniela Adriana Ion, Ioana Anca Badarau, Catalin Mihai Popescu, Raluca Popescu, Cristiana Popp, Mirela Cioplea, Luciana Nichita, Ionela Hulea, A
Analytical Cellular Pathology.2019; 2019: 1. CrossRef
Towards understanding the mechanisms of actions of carcinoembryonic antigen‐related cell adhesion molecule 6 in cancer progression
Balsam Rizeq, Zain Zakaria, Allal Ouhtit
Cancer Science.2018; 109(1): 33. CrossRef
Lung Cancers: Molecular Characterization, Clonal Heterogeneity and Evolution, and Cancer Stem Cells
Ugo Testa, Germana Castelli, Elvira Pelosi
Cancers.2018; 10(8): 248. CrossRef
SOX2 knockdown inhibits the migration and invasion of basal cell carcinoma cells by targeting the SRPK1‑mediated PI3K/AKT signaling pathway
Zhuo‑Ran Li, Yong Jiang, Jian‑Zhong Hu, Yang Chen, Quan‑Zhong Liu
Oncology Letters.2018;[Epub] CrossRef
Association of SOX2 and Nestin DNA amplification and protein expression with clinical features and overall survival in non-small cell lung cancer: A systematic review and meta-analysis
Qingbao Li, Fang Liu, Yuan Zhang, Lei Fu, Cong Wang, Xuan Chen, Shanghui Guan, Xiangjiao Meng
Oncotarget.2016; 7(23): 34520. CrossRef

11,942 View
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