| 위암 세포주에 대한 항암제 5-FU 와 Alpha-interferon 의 상승적 항암효과 |
| 김삼용, 박상준, 김종숙, 최지영, 윤환중, 전의건, 조덕연, 배진선 |
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| Synergistic Antitumor Effects of 5-Fluorouracil ( 5-FU ) and Alpha-interferon 2a Gastric Cnancer Cells |
| Sam Yong Kim, Sang Jun Park, Jong Suk Kim, Jee Young Choi, Hwan Jung Yun, Eui Gun Chun, Deog Yeon Jo, Chin Sun Bae |
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| ABSTRACT |
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Many in vitro studies or clinical trials reported synergistic effect of the combination of 5-FU and alpha-interferon 2a(IFNa-2a) in patients with colorectal cancer, urinary bladder cancer, and esophageal cancer. There have been few reports on the effects of the combination of 5-FU and IFN against gastric cancer ce11 lines. This study was conducted to investigate the com- bined cytotoxic effects of 5-FU or cisplatin with IFNa-2a against two gastric cancer cell lines. SNU-1 and SNU-l6 cell lines were treated with a serial concentrations of 5-FU plus IFNa-2a (5000: 1, molar ratio) and cisplatin plus IFNa-2a (400: 1, molar ratio). Cytotoxicity was determined using a tetrazolium-based colorimetric (MTT) assay. The combination effect of two drugs was evaluated by the median effect principle after Chou et aL Calculation of Assay-AUC was done after Park et aL In SNU-1 cell line, combination of 5-FU and IFNa-2a showed a synergistic effect (combination index, CI<1). Assay AUC of 5-FU was markedly reduced com- parable to clinically achievable-AUC in all combinations. In SNU-16 cell line, combination of 5- FU or cisplatin with IFNa-2a showed no synergism (CI> 1). But inhibitory concentration (IC ) value was markedly reduced. So 5-FU or cisplatin activity were markedly enhanced by IFNa- 2a, especially at low doses. |
| Key words:
5-FU, IFNa-2a, Synergistic antitumor effects, Gastric cancer |
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